# Intraoperative Kit for AuNP-Tissue Grafts

> **NIH NIH R41** · G5 BIOLOGICAL INNOVATIONS L.L.C. · 2022 · $178,101

## Abstract

Project Summary/Abstract
The proposed technology is an intraoperative kit comprised of a liquid solution which will conjugate gold
nanoparticles (AuNPs) to tissue grafts within minutes. Tissue grafts, such as those used in ACL reconstruction
surgery, rarely achieve 100% graft assimilation, thereby leading to a host of problems. A potential solution is
to modify the grafts in order to enhance integration and reduce inflammation and thus improve clinical
outcomes. The innovation of this technology is the development of the intraoperative kit to attach AuNPs to
grafts in surgical suite settings. These grafts can be autografts or commercially available allografts, xenografts,
or any other natural materials such as collagen or 3D printed tissues. The specific aims of the project will
validate the concept of an intraoperative AuNP-tissue conjugation method. Two specific aims will be rigorously
pursued, which include 1) optimizing the intraoperative kit method by producing AuNP grafts that reduce
inflammation while increasing cellular proliferation via in vitro studies and 2) validating the inflammation
modulation ability and cellular proliferation of the modified grafts in an ACL reconstruction in vivo model.
Currently, there are no commercial technologies that modify biologic grafts in order to promote remodeling and
reduce inflammation. The significance of this research is the development of an intraoperative method to modify
biological grafts to improve clinical performance. The PI and sub-contract PI have the expertise to pursue the
aims of this STTR Phase 1 proposal; they have years of experience developing nanomaterial-tissue
bionanocomposites and have been involved with several start-up companies.

## Key facts

- **NIH application ID:** 10598225
- **Project number:** 1R41AR082170-01
- **Recipient organization:** G5 BIOLOGICAL INNOVATIONS L.L.C.
- **Principal Investigator:** David Grant
- **Activity code:** R41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $178,101
- **Award type:** 1
- **Project period:** 2022-09-20 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10598225

## Citation

> US National Institutes of Health, RePORTER application 10598225, Intraoperative Kit for AuNP-Tissue Grafts (1R41AR082170-01). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10598225. Licensed CC0.

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