# Cell Technologies Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2023 · $1

## Abstract

ABSTRACT 
Overview: The Cell Technologies Shared Resource (CTSR) supports basic and translational research projects 
for University of Colorado Cancer Center (UCCC) members. CTSR produces hybridomas/monoclonal antibodies 
to enhance basic research and preclinal studies and makes recombinant proteins for mechanistic and structural 
studies on proteins. To address the growing importance of organoid-based studies as culture models that better 
mimic in vivo biology, the CTSR offers training and fee-for-service organoid services. We provide multiple 
platforms and analytic modules for real time live-cell imaging of cultured cells and organoids to enhance analysis 
of cancer cell biology. Services and Expertise: CTSR offers over 500 authenticated cell lines enhancing Rigor 
in Research on our campus, and has over 25 years of experience in generating hybridomas and in producing 
purified monoclonal antibodies and recombinant proteins to support basic, translational and preclinical research. 
In this funding period, the SR added state-of-the-art services, training, and consulting focused on use of organoid 
technologies. Equipment. The CTSR has all required equipment to support isolation of hybridomas, production 
and purification of monoclonal antibodies, and of recombinant proteins from mammalian or insect cells, and 
general cell culture. A large capacity liquid nitrogen system stores established cell lines, hybridomas, and other 
biological samples. The Sartorius IncuCyte imaging system was updated in 2020 and offers real time imaging of 
living cells to quantitate cell proliferation, apoptosis, cell migration, chemotaxis, cell cycle changes, spheroid 
cultures, and organoid cultures in an automated, high throughput manner. A Mantis liquid handling system and 
Olympus IX83 with spinning disk imaging system support organoid culture projects. Management: CTSR is a 
blended SR managed by UCCC and the School of Medicine. Currently CCSG funding represented 19% of the 
annual operating budget. The remaining support is from user fees (40%), institutional support (32%), and other 
center grants (9%). Use of Services: Since July 2016, 244 investigators have used the services. Forty-nine 
percent of users (119) were UCCC members, representing all four UCCC Programs and resulting in 57 peer- 
reviewed cancer-relevant publications. Consultation and Education: CTSR provides seminars to inform 
investigators of the research potential in live cell imaging, and advances in organoid culture; and offers a three- 
week course on organoids and stem cells for graduate students. The CTSR also participates in a Rigor in 
Research course offered to graduate students. On-site office hours with a Technical Service Representatives for 
the IncuCyte systems are offered; and the Directors and Staff routinely consult with investigators on projects to 
ensure successful outcomes. Future Plans: Organoid-based technologies are advancing rapidly offering new 
opportunities for basic...

## Key facts

- **NIH application ID:** 10599146
- **Project number:** 5P30CA046934-35
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** RICHARD D SCHULICK
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1
- **Award type:** 5
- **Project period:** 1997-04-04 → 2027-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10599146

## Citation

> US National Institutes of Health, RePORTER application 10599146, Cell Technologies Shared Resource (5P30CA046934-35). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10599146. Licensed CC0.

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