# Flow Cytometry Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2023 · $1

## Abstract

ABSTRACT 
Overview: The Flow Cytometry Shared Resource (FCSR) has served as a cornerstone for UCCC research 
since 1988, supporting basic, translational, and clinical cancer investigators seeking to use cytometry-based 
technologies (flow cytometry, FACS and mass cytometry or CyTOF). These technologies allow the identification, 
quantification and purification of cells based on gene and protein expression. Services and Expertise: The 
FCSR provides the equipment and staff expertise to support application of, and education in, a range of 
cytometry-based methods. The FCSR mission is supported by a highly knowledgeable staff with >80 years 
combined expertise in cytometry-based research. Equipment: The FCSR houses 4 cell sorters, 3 conventional 
flow cytometers, 1 spectral flow cytometer, and a Helios mass cytometer, enabling cytometry-based research 
from simple to high-end analysis. Management: The FCSR is a Cancer Center-managed SR, led by Clambey 
(THI), an immunologist who frequently leverages flow and mass cytometry-based studies to investigate 
mechanisms of immune control or failure; and FCSR manager, Acosta, who has >21 years of expertise in flow 
cytometry in academia and industry. Use of Services: Since 2016, the FCSR has provided support to 137 UCCC 
researchers, accounting for 67% of total revenues and resulting in 125 cancer-related publications. FCSR 
services are used by UCCC members from all four programs. Operating Budget: CCSG funds represent 15% 
of the operating budget, with the remainder from chargebacks (60%) and institutional support (25%). Innovation 
and Developments: The FCSR continues to innovate and expand services to address evolving user needs and 
new opportunities in cytometry-based research. In the most recent funding period, the FCSR added two new 
services - investigator-operated cell sorting and spectral flow cytometry; established a CyTOF antibody bank to 
reduce the barrier to entry for investigators interested in using CyTOF; and initiated remote investigator-operated 
flow cytometer access to ensure continued service during the COVID-19 pandemic. Consultation and 
Education: The FCSR uses its experience and knowledge to consult with, and educate, investigators in the use 
of cytometry-based research, conducting >800 consultations and >300 training session since 2016. Education 
is further promoted through one-on-one meetings, user groups, and seminars. Future Directions: The FCSR 
will continue to provide robust support for cytometry-based research by UCCC members, through acquisition 
and deployment of cutting-edge equipment, development of highly experienced staff, and an ongoing 
commitment to user education in cytometry-based research and new research opportunities. In order for these 
services to remain competitive, the FCSR will further: i) update cell sorting capacity through acquisition of a high- 
parameter, user-operated cell sorter with spectral sorting capacity and a built-in biosafety cabinet, a...

## Key facts

- **NIH application ID:** 10599155
- **Project number:** 5P30CA046934-35
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** RICHARD D SCHULICK
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1
- **Award type:** 5
- **Project period:** 1997-04-04 → 2027-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10599155

## Citation

> US National Institutes of Health, RePORTER application 10599155, Flow Cytometry Shared Resource (5P30CA046934-35). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10599155. Licensed CC0.

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