ABSTRACT: The overall goal of this proposal is to examine the role of three critical components of graduate training in determining successful outcomes. We hypothesize that important factors which impact career outcomes and job satisfaction include participation in: a) professional development (PD), b) mental health and wellness of trainees, and c) mentor-mentee match. We will also measure and account for the potential explanatory effects of existing individual factors including intersectional identities (underrepresented status in the sciences; e.g., race, gender, LGBTQIA, first generation). Collectively, this evidence-based evaluation will be critical as institutions decide how to focus limited resources to improve the training environment and effectively train the biomedical workforce and future scientific leaders. The three specific aims encompass the following: Determine the impact of professional development training on career outcomes (AIM 1); Measure the impact of professional development participation and engagement on trainee mental health (AIM 2); and determine the impact of mentor-mentee match on scientific leadership career outcomes (AIM 3). In addition, this supplement will support the professional development of an early career investigator by transitioning research areas of expertise to include higher education research methods and experience. To enable a shift to independent research in this area, the candidate will complete training in the following areas: 1) interdisciplinary education research methods (qualitative and quantitative); 2) advanced statistical analyses, research design/methods, & responsible conduct of research; 3) leadership and project management training; 4) grant and manuscript writing; and 5) training in program evaluation and administration. This will advance the aims of the SCISIPBIO study to allow for the examination of additional factors that may impact trainee mental health outcomes and to expand the investigation of mentor-mentee-match to examine outcomes across national data, while advancing the career trajectory of an outstanding future scholar in biomedical workforce development, education, and training.