Project Summary No woman should die from cervical cancer. We have the technical, medical and policy tools and approaches to eliminate it. Yet, one woman dies of cervical cancer every two minutes. Cervical cancer is the third leading malignancy among women in the world, after breast and colorectal cancer. Cervical cancer is also one of the tumors in which the most glaring disparities exist worldwide. The dramatic disparity in incidence rates between high- and low-income countries is due primarily to differential access to effective screening and pre- cancer, or preventive, treatment; similar disparities also exist within countries. Cervical cancer incidence and mortality has been higher for decades among Latinas in the United States (US) [8]. A recent study found that cervical cancer incidence is 32 percent higher among Latinas in the 50 states and 78 percent higher in the US territory of Puerto Rico, compared to non-Latina white women. The screening rates among Latinas is also. The World Health Organization (WHO) 2021 guidelines for treatment of cervical intraepithelial neoplasia 2-3 and screen-and-treat strategies to prevent cervical cancer, includes DNA methylation in the screening algorithm. A variety of alternative algorithms are being used for cervical cancer screening in low-income populations, which include oncogenic HPV testing, visual inspection with acetic acid, and self-collected vaginal swabs. HPV testing is an excellent alternative to cytology for cervical cancer screening. However, HPV tests identifies women at risk for cervical cancer, but not those HPV-positive women who are most likely to have, or to develop in the near future, significant disease requiring treatment. Testing DNA biomarkers in self collected vaginal swabs can increase cancer screening rates and lead to a reduction in cervical cancer disparities among Latinas in the US. In this Fast Track SBIR project, we propose to demonstrate the feasibility for the commercialization of a precision methylation test in self-collected swabs among Latinas, using the Precision Cervical Health Promotion program using the CervicalMethDx test, to stratify selfcollected swab of HPV+ patients for high risk of cervical cancer. The CervicalMethDx test will enable identification of HPV+ women at clinical risk for advancement from low-grade squamous intraepithelial lesions (LSIL) to Cervical Intraepithelial Neoplasia grade 3 (CIN3) in self-collected samples. We are partnering with Salud Integral en la Montaña, PathAdvantage Clinical laboratory and, David Sidransky’s research laboratory to implement the Precision Cervical Health Promotion and optimize CervicalMethDx Test in self-collected samples, to introduce precision epigenetic services to Latinas in the US and the territory of Puerto Rico.