# Development of a high precision and high order DNA counting platform

> **NIH NIH R44** · ENUMERIX, INC. · 2023 · $1,080,705

## Abstract

PROJECT SUMMARY/ABSTRACT
Enumerix has developed a cost-effective method to perform high order DNA counting at high precision
called direct count PCR (dcPCR). dcPCR uses a spin column method that generates millions of
partitions directly into PCR tubes for target amplification, using standard laboratory centrifuge and
thermal cyclers, followed by an ultra-rapid method to count up to 1 million copies of DNA directly from
the same PCR tube. We have demonstrated that our technology far exceeds the precision of digital
PCR, and can perform at a DNA counting range akin to quantitative PCR and next generation
sequencing, which makes it a suitable next-generation DNA counting platform. For this proposed Phase
II project we plan to further develop our dcPCR platform with the goal to distribute the technology to
end-users to replace quantitative PCR and digital PCR.

## Key facts

- **NIH application ID:** 10603953
- **Project number:** 1R44GM149104-01
- **Recipient organization:** ENUMERIX, INC.
- **Principal Investigator:** Eleen Yee Lam Shum
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1,080,705
- **Award type:** 1
- **Project period:** 2023-04-01 → 2024-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10603953

## Citation

> US National Institutes of Health, RePORTER application 10603953, Development of a high precision and high order DNA counting platform (1R44GM149104-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10603953. Licensed CC0.

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