Conformational Energetics and Heterogeneity to Reveal Gating Mechanisms of TRPV and TRPM Ion Channels

NIH RePORTER · NIH · R35 · $66,204 · view on reporter.nih.gov ↗

Abstract

Project Summary/Abstract This request for an administrative supplement to promote diversity will allow Dr. Teresa M Swanson to train as a postdoctoral scholar in the Gordon lab. She will work on the TRPM2 project in the scope of the parent R35 grant. This project addresses the molecular mechanisms governing Ca2+- and ADP Ribose-driven conformational rearrangements in TRPM2 channels. Dr. Swanson will train directly under Dr. Gordon in the experimental methods required: transition metal ion FRET using both steady-state and time-resolved fluorescence measurements; using amber codon suppression to introduce unnatural amino acids; and patch-clamp fluorometry. Work on this project will provide Dr. Swanson with excellent training to advance her career. She will also participate in career development opportunities and programs to enhance diversity of the biomedical workforce, as described in the training plan.

Key facts

NIH application ID
10605108
Project number
3R35GM145225-01S1
Recipient
UNIVERSITY OF WASHINGTON
Principal Investigator
Sharona E Gordon
Activity code
R35
Funding institute
NIH
Fiscal year
2022
Award amount
$66,204
Award type
3
Project period
2022-04-01 → 2027-03-31