# Administrative Supplement for Combining Experiments and Computer Simulation to Improve the Stem Cell Differentiation Process

> **NIH NIH R15** · UNIVERSITY OF TEXAS ARLINGTON · 2022 · $315,811

## Abstract

ABSTRACT
The overall goal of this work is to experimentally observe stem cell differentiation, adipogenesis, of single cells to
gain a more accurate model of the changes in mechanobiology over time. The high-speed simulation model of
adipogenesis is currently based on observations of averaged ensemble properties of stem cell cultures. Because
the model is of a single cell, its accuracy and predictive capability can be improved by obtaining more data on the
changes in stem cell properties for single cells that can be tracked over time. An incubator will be developed as
part of this grant to sustain stem cells under a microscope for at least 15 days, which will allow photographs at
regular intervals of the morphology of the cell and nuclear membranes. However, time-dependent data on many
cell properties is still difficult to obtain because the process of dyeing the cell to examine the state of its compo-
nents, or probing its properties with an optical tweezer, typically kills the cell. Thus, the cells will be sacrificed
after 15 days, and the high-speed simulation will be used work backward to estimate the initial properties related
to successful and unsuccessful adipogenesis. Comparing successful and unsuccessful differentiation will yield
biomarkers that provide early indication of successful adipogenesis, as proposed in Aim 1 of the parent grant.
These additional single stem cell experiments will complement the data obtained from the ensemble experiments
proposed in the parent grant.

## Key facts

- **NIH application ID:** 10606343
- **Project number:** 3R15EB030842-01S2
- **Recipient organization:** UNIVERSITY OF TEXAS ARLINGTON
- **Principal Investigator:** Alan Paul Bowling
- **Activity code:** R15 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $315,811
- **Award type:** 3
- **Project period:** 2021-09-01 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10606343

## Citation

> US National Institutes of Health, RePORTER application 10606343, Administrative Supplement for Combining Experiments and Computer Simulation to Improve the Stem Cell Differentiation Process (3R15EB030842-01S2). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10606343. Licensed CC0.

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