Summary/Abstract At the most fundamental level, physical and chemical laws such as thermodynamics and mass action govern the operations of a living cell. At the functional level, however, when, where and at what rate a biomolecule executes its function through its binding to a partner or its enzymatic actions is stochastic, and can only be described in probabilistic instead of deterministic terms. At this level, the integration between experiments and theories becomes extremely important, because new theories need to be developed to explain stochastic experimental observations and guide further experimental explorations, and experiments need to be conducted to test theoretical predications and establish biological significance. The importance of stochastic physics in biology has been recognized by the growing research community and publications in premier journals. The 2023 GRC Stochastic Physics in Biology aims to bridge stochastic physical theories with biological experiments by bringing together an outstanding and diverse group of physicists, mathematicians, engineers and biologists at the forefront of research. It will address emerging challenges in understanding complex behaviors of biological systems using principles and theories in stochastic physics. A carefully designed conference plan with nine scientific sessions, four poster sessions, one Power Hour, and three social hours will foster a stimulating environment for participants and serve as a vigorous intellectual hub for the growing community. The conference will benefit the mission of NIBIB by providing a fertile scientific ground to accelerate the application of biophysical and biomedical technologies to human health.