Diaz-Campos et al. Vet-LIRN Capacity-Building Project Facilitating antimicrobial stewardship with an educational video series approach Abstract National and international organizations addressing the threat of antimicrobial resistance have prioritized the education on antimicrobial resistance and stewardship directed to prescribers, other healthcare providers, and animal owners (patients). Additionally, these organizations recommend educating each of these groups when implementing antimicrobial stewardship programs. The expansion of our Ohio State University Buckeye ASP into veterinary private practices in Ohio is in progress, and one of the core components of our program is education and training. We hypothesized that the creation and dissemination of educational videos on antimicrobial use, resistance, and stewardship targeting both lay and scientific audiences will result in more appropriate antimicrobial use in veterinary medicine and reduce selection pressure favoring resistant bacterial strains. The aims of this proposal are: 1) to create and disseminate educational videos on antimicrobial use, resistance, and stewardship topics appropriate for a lay audience including owners of companion animals; and 2) to create and disseminate educational videos on antimicrobial use, resistance, and stewardship topics appropriate for a scientific audience including veterinary medical professionals. We expect to reach out to antimicrobial-prescribers (veterinarians), other healthcare veterinary providers, and animal owners through the creation of educational videos medical which will result in promoting the judicious use of antimicrobials and commitment to other antimicrobial stewardship efforts. These videos will allow us to extend education and training to people that are not members of our program. In addition, the creation of these videos will support the mission of the Vet-LIRN network and the CVM-FDA.