# Sequencing Core

> **NIH NIH U19** · LA JOLLA INSTITUTE FOR IMMUNOLOGY · 2023 · $189,432

## Abstract

Sequencing Core Summary
The mission of the HIPC program at LJI is to use hypothesis-free approaches to define comprehensive molecular
profiles of T cell subsets in the context of lung pathogens. The areas of interest cover an extremely wide spectrum
of “omics” analysis including: (i) analysis of global gene expression (bulk and single-cell RNA-seq), (ii) analysis
of T cell receptors high variable sequences repertoire by bulk or single-cell TCR-seq, (iii) analysis of changes in
global epigenetics profiles (genome-wide enhancer profiles) using single-cell ATAC-seq, in T cell memory
subsets from patients infected and/or vaccinated against, SARS-CoV-2, Mycobacterium tuberculosis (Mtb), and
other lung pathogens. All these “omics” applications rely on standardized and well-validated high-throughput
Next-Generation Sequencing (NGS) methods – a key service provided by this Core.
The Sequencing Core will support all NGS activities for all the three projects involved in this program. This will
be facilitated by the extensive genomics expertise of its personnel and the availability of cutting-edge
technologies and automated platforms to generate highly reproducible and robust sequencing data from a large
number of samples. Practically, the Sequencing Core will interface directly with all three Projects and provide a
standardized and quality-controlled pipeline consisting of receiving samples, sorting cells, preparing RNA/DNA,
preparing libraries for high-throughput NGS, and transferring the resulting data to the Data Management and
Analysis Core for subsequent analysis.

## Key facts

- **NIH application ID:** 10619607
- **Project number:** 5U19AI118626-09
- **Recipient organization:** LA JOLLA INSTITUTE FOR IMMUNOLOGY
- **Principal Investigator:** Gregory Seumois
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $189,432
- **Award type:** 5
- **Project period:** 2015-06-15 → 2027-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10619607

## Citation

> US National Institutes of Health, RePORTER application 10619607, Sequencing Core (5U19AI118626-09). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10619607. Licensed CC0.

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