# Genomic and Microenvironment Analysis of HIV-Associated Diffuse Large B-cell Lymphoma (Immuno/Microenvironment)

> **NIH NIH P30** · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · 2022 · $249,999

## Abstract

PROJECT SUMMARY/ABSTRACT
This proposal addresses the high priority statement related to “HIV-associated comorbidities, coinfections, and
complications.” This study will be addressing this high priority requirement from two aspects, including the study
of co-infection by EBV and HIV, as well as the molecular differences between EBV+ and EBV- diffuse large B-
cell lymphoma in HIV+ patients.
HIV-associated diffuse large B-cell lymphoma (HIV+/DLBCL) remains an understudied disease and is one of the
leading causes of death in persons living with HIV on anti-retroviral therapy. Although there have been significant
strides taken with de novo DLBCL in terms of molecular classification, HIV+/DLBCL has lagged behind,
particularly because it was originally excluded from most large studies. While approximately 30% of these tumors
can be EBV-positive, potentially serving as a driving mechanism for lymphomagenesis, the molecular
pathogenesis in the majority of cases have not been comprehensively studied. Additionally, the tumor
microenvironment (TME) plays an important role in lymphomas related to prognosis and therapy, but this area
has not been investigated in HIV+/DLBCL. We hypothesize that by investigating the genomic landscape and
TME of HIV+/DLBCL, we will identify key cellular vulnerabilities and pathways that can provide insights for
tailoring novel therapies for patients with HIV+/EBV-positive versus HIV+/EBV-negative DLBCL. Utilizing our
multi-institutional collaboration, we plan to evaluate the 1) whole “omics” of HIV+/DLBCL as well as 2) investigate
the TME of HIV+/DLBCL using multispectral immunofluorescence in conjunction with spatial gene expression
data, EBV-status, and genomic data. 3) We will utilize the Lymphoma and Leukemia Molecular Profiling Project
(LLMPP) with their established pathology review and bioinformatics pipeline to facilitate this study which will
generate important data that we and other investigators around the world can use in future years to generate
new hypothesis, design targeted treatments and trials for this aggressive disease.

## Key facts

- **NIH application ID:** 10620074
- **Project number:** 3P30CA033572-39S3
- **Recipient organization:** BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
- **Principal Investigator:** STEVEN Terry ROSEN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $249,999
- **Award type:** 3
- **Project period:** 2022-09-01 → 2022-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10620074

## Citation

> US National Institutes of Health, RePORTER application 10620074, Genomic and Microenvironment Analysis of HIV-Associated Diffuse Large B-cell Lymphoma (Immuno/Microenvironment) (3P30CA033572-39S3). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10620074. Licensed CC0.

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