# Flow Cytometry Core

> **NIH NIH P30** · UNIVERSITY OF SOUTHERN CALIFORNIA · 2023 · $229,860

## Abstract

PROJECT SUMMARY – Flow Cytometry and Immune Monitoring Shared Resource
The mission of the USC Norris Comprehensive Cancer Center (NCCC) Flow Cytometry and Immune Monitoring
Shared Resource (FCIM) is to provide advanced multi-parameter flow cytometry, cell sorting capabilities, and
immune monitoring services for advancing basic, translational, and clinical research with options for full-service
technical support for cancer clinical trial immune-monitoring correlative studies. NCCC Administration oversees
FCIM with Martin Kast PhD (continuing in the role) and Rong Lu PhD (new in the role in 2015) as the faculty Co-
Directors. Kast and Lu have extensive and complementary scientific expertise; they meet monthly to provide
executive oversight for core services and to promote user access and satisfaction. In response to annual NCCC
surveys of Shared Resources (SR) and feedback from the NCCC External Advisory Board, FCIM significantly
enhanced services and new equipment during the current grant cycle, including: 1) expanded sorting and high
throughput analysis capabilities with 3 new instruments offering more available colors, 2) increased post-sort
viability, 3) added services for mass cytometry (CyTOF)-assisted projects; and 4) lowered costs due to improved
efficiencies. In the next grant cycle, based on user feedback, FCIM will expand its services to include T cell
receptor (TCR) clonality determination and other single cell omics using a newly acquired Illumina MiSeq System
in the Molecular Genomics SR and spatial immune profiling with the recently acquired Vector Polaris system in
the Translational Pathology SR. During the current grant period (2015-2020), 70 NCCC members representing
all five programs utilized FCIM, resulting in 53 publications and $15.9M in direct cost grant funding as a direct
result of usage. The anticipated annual budget of FCIM in the first year of the next grant cycle is $632,453, yet
the CCSG request is $143,215. Accordingly, FCIM leverages extensive institutional and recharge support and
seeks only 23% from CCSG funds.

## Key facts

- **NIH application ID:** 10620222
- **Project number:** 5P30CA014089-47
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **Principal Investigator:** WIJBE MARTIN KAST
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $229,860
- **Award type:** 5
- **Project period:** 1996-12-01 → 2026-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10620222

## Citation

> US National Institutes of Health, RePORTER application 10620222, Flow Cytometry Core (5P30CA014089-47). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10620222. Licensed CC0.

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