# Role and mechanisms of excretion in manganese neurotoxicity

> **NIH NIH R01** · UNIVERSITY OF TEXAS AT AUSTIN · 2022 · $72,620

## Abstract

ABSTRACT
 Work on this diversity supplement will (1) test the impact of early-life inhibition of manganese excretion on
dopamine metabolism in the brain using microdialysis; and (2) provide career development opportunities for
Stephanie Grant. Our specific rationale is below.
 In the parent grant, we had proposed to interrogate the neurotoxic effects of Mn in the liver or intestine-
specific Slc30a10 knockout or knockin strains using 4 approaches: (1) neurobehavioral assays to assess Mn-
induced changes in skilled motor function and locomotor/exploratory activity; (2) inductively-coupled plasma
mass spectrometry (ICP-MS) metal analyses to quantify brain Mn levels; (3) microscopy to assess neuronal
degeneration, neuroinflammation, and apoptosis; and (4) tissue neurochemistry to assess striatal levels of
catecholamines. To increase the impact and significance of our studies, we now propose to add one more
approach to quantify Mn neurotoxicity in a selected group of animals through this diversity supplement – in vivo
microdialysis to quantify evoked dopamine release. Inclusion of the microdialysis study through this diversity
supplement will (1) substantially increase the significance of the findings of the parent proposal; and (2) provide
Stephanie with a high-impact, independent project that fosters her scientific and career development.

## Key facts

- **NIH application ID:** 10625703
- **Project number:** 3R01ES031574-03S1
- **Recipient organization:** UNIVERSITY OF TEXAS AT AUSTIN
- **Principal Investigator:** Somshuvra Mukhopadhyay
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $72,620
- **Award type:** 3
- **Project period:** 2020-07-03 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10625703

## Citation

> US National Institutes of Health, RePORTER application 10625703, Role and mechanisms of excretion in manganese neurotoxicity (3R01ES031574-03S1). Retrieved via AI Analytics 2026-06-08 from https://api.ai-analytics.org/grant/nih/10625703. Licensed CC0.

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