# Imaging and Image Analysis Core

> **NIH NIH P01** · UNIVERSITY OF COLORADO DENVER · 2023 · $225,514

## Abstract

ABSTRACT
The Imaging and Image Analysis Core will perform mRNAs spatial and cellular localization and expression levels
analyses, immunofluorescence, as well as quantitative label free fibrosis imaging on human lung tissues-based
investigations for each one of the Projects. Samples from specific functional lung regions of extensively
phenotyped IPF lungs represent a unique resource that will benefit all three Projects in the Program, and these
specimens will need to be carefully stained and imaged for mRNA and protein identification and quantification
accumulation to assess ER stress, autophagy, UPR, cell senescence, apoptosis, and fibrosis. Centralization of
advanced staining and microscopic imaging will also benefit all Projects by providing quality-controlled data for
Project-specific investigations, thus improving the opportunity for inter-project collaboration and integration. This
Core is therefore an essential integrative component of the Program.
The Aims of the Imaging and Image Analysis Core include:
 • Design staining combinations and perform regions selection for whole transcriptome analysis with
 Nanostring GeoMX Digital Spatial Profiler.
 • Perform RNAscope in situ hybridization and microscopic imaging of IPF and control lung tissue to
 complement, validate, and contextualize the transcriptional profiles obtained by RNA-seq analyses and
 to address other project-specific needs.
 • Perform label-free second harmonic generation imaging of fibrillar collagens for fibrosis quantification.
 • Perform analyses and quantification of microscopic images.
 • Provide Digital Whole Slide Images storage in conjunction with the Data Management and Analysis Core
 (Core D).
The Imaging and Image Analysis Core will be led by Evgenia Dobrinskikh, PhD who will coordinate, overview,
and perform services for the Scientific Projects and coordinate with other Research Cores.

## Key facts

- **NIH application ID:** 10627596
- **Project number:** 1P01HL162607-01A1
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Evgenia Dobrinskikh
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $225,514
- **Award type:** 1
- **Project period:** 2023-07-01 → 2028-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10627596

## Citation

> US National Institutes of Health, RePORTER application 10627596, Imaging and Image Analysis Core (1P01HL162607-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10627596. Licensed CC0.

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