# Cas9 RNP targeting cis-regulatory elements in lymphocytes

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2022 · $78,366

## Abstract

Project Summary
 CRISPR tools for genome editing have revolutionized and accelerated the functional interrogation of coding
genes in model animal systems and in human somatic cells, including primary lymphocytes that play critical
roles in the immunopathology of allergy and asthma. We propose to adapt and optimize these tools for miRNA-
directed pathway discovery. First, will create pooled retroviral CRISPR libraries for targets that we have
empirically identified for miR-29, a miRNA with critical functions in both T cells and B cells. Second, we will
extend next generation transfection-based CRISPR systems to target cis-regulatory sequences that do not
code for protein, but mediate regulatory interactions between miRNAs and target mRNAs. Success in these
aims will enable detailed mechanistic analysis of miRNA:target networks that govern immune responses in
asthma and allergy. A second important goal of this proposal is to support the career and scientific
development of Celeste Garza, a post-baccalaureate researcher gaining research experience as a junior
specialist in the Ansel laboratory. The proposed experiments build on skills she has learned in the Ansel lab,
and will provide the opportunity for her to develop a research narrative and scientific achievements as prepares
to apply for PhD programs in the coming year.

## Key facts

- **NIH application ID:** 10630567
- **Project number:** 3R01HL109102-12S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Karl Mark Ansel
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $78,366
- **Award type:** 3
- **Project period:** 2011-08-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10630567

## Citation

> US National Institutes of Health, RePORTER application 10630567, Cas9 RNP targeting cis-regulatory elements in lymphocytes (3R01HL109102-12S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10630567. Licensed CC0.

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