# Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

> **NIH NIH R01** · COLORADO STATE UNIVERSITY · 2022 · $370,124

## Abstract

PROJECT SUMMARY / ABSTRACT
This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements
for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL-
affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito-
endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award
through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the
composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti.
The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides
biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life
cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection
and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent
award data outcomes by providing additional insight into the metabolic state of the vector following infection with
all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be
applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts
synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods
for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.

## Key facts

- **NIH application ID:** 10631510
- **Project number:** 3R01AI151166-03S2
- **Recipient organization:** COLORADO STATE UNIVERSITY
- **Principal Investigator:** Rushika Perera
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $370,124
- **Award type:** 3
- **Project period:** 2020-03-01 → 2025-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10631510

## Citation

> US National Institutes of Health, RePORTER application 10631510, Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement (3R01AI151166-03S2). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10631510. Licensed CC0.

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