Project Summary/Abstract Stanford is in dire need of a modern triple quadrupole mass spectrometer to serve the needs of our campus community of biomedical researchers. The proposed Agilent 6495C LC/MS system will replace an 11-year-old workhorse instrument at Stanford University Mass Spectrometry, the campus-wide shared resource. This premier bioanalytical platform will bring both new capabilities and greater bandwidth to positively impact the goals of NIH-funded researchers. The instrument performance requirements for modern research have long eclipsed the usefulness of our existing instruments in terms of sensitivity and linearity as well as capacity for high-demand applications in NIH-funded biomedical research projects. Our users require the capability to observe and quantify a greater number and diversity of analytes simply not obtainable on existing platforms, and to do so at larger sample scale to address their growing biomedical research needs. The Agilent 6495C LC/MS will support numerous existing projects with current NIH funding which are either unable to proceed due to lack of capability on campus or greatly delayed due to lack of bandwidth with sufficient performance to meet their application needs. Stanford University Mass Spectrometry has all of the technical expertise, physical infrastructure, and administrative processes already in place and ready to be unlocked upon instrument installation. The addition of this instrument would immediately accelerate numerous key research areas, including heart disease, cancer, neurodegeneration, aging, arthritis, diabetes, and sensory impairment diseases.