# JEOL 2100PLUS(CR)

> **NIH NIH S10** · UNIVERSITY OF GEORGIA · 2023 · $1,190,000

## Abstract

Project Summary/Abstract
Cryogenic Transmission Electron Microscopy (cryoTEM) has emerged as a powerful
approach to acquiring structural information from proteins, subcellular organelles, and other
biomacromolecules and their assemblies. This proposal seeks funding to bring cryoTEM
capabilities to the University of Georgia for the purpose of facilitating NIH-supported
research related to cell organelle biogenesis, the biochemistry of metabolism and gene
expression, the mechanisms of diverse human diseases and disorders, antibody
therapeutics, antivirals, treatments for infectious diseases, and fundamental structural and
cellular biology. The proposed equipment includes a cryo-capable, 200 kV transmission
electron microscope (JEOL 2100PLUS) with a direct electron detection device (Direct
Electron DE16ER camera) and the essential equipment needed for sample vitrification and
handling (a Leica EM GP2 automatic plunge freezer, and a Gatan Elsa cryo sample holder
with turbo benchtop pumping station). This multipurpose cryoTEM system will support the
needs of NIH-funded investigators at the University of Georgia (UGA) for (1) the preparation
and screening of vitrified samples; (2) data collection for single particle analysis,
tomographic reconstructions, and micro-electron diffraction; and (3) improved conventional
TEM imaging. This equipment will be installed at the Georgia Electron Microscopy (GEM)
core facility in custom-designed laboratory space, and it will be operated and maintained by
GEM’s staff. By establishing campus-level access to cryoTEM instrumentation, services,
expertise, and hands-on training, this project will enable numerous biomedical researchers to
incorporate cryoTEM methods into their research programs. UGA’s vibrant, NIH-supported
research groups include five major users of cryoTEM, eight minor users of cryoTEM, and
additional investigators who will be future cryoTEM practitioners.

## Key facts

- **NIH application ID:** 10632787
- **Project number:** 1S10OD034282-01
- **Recipient organization:** UNIVERSITY OF GEORGIA
- **Principal Investigator:** Tina Salguero
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1,190,000
- **Award type:** 1
- **Project period:** 2023-09-08 → 2024-09-07

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10632787

## Citation

> US National Institutes of Health, RePORTER application 10632787, JEOL 2100PLUS(CR) (1S10OD034282-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10632787. Licensed CC0.

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