# A SARS-CoV-2 NFC ePAD Biosensor

> **NIH NIH R01** · COLORADO STATE UNIVERSITY · 2022 · $58,053

## Abstract

Proposed here is the development of an improved electrochemical sensor for detection of
the SARS-CoV-2 nucleocapsid protein using thermoplastic electrodes (TPEs). The need for faster
testing amid the COVID-19 pandemic brought about several alternatives to RT-qPCR testing for
viral infections. However, current rapid tests are lacking in sensitivity (lateral flow assays and
other paper based tests) or expensive (gold based electrochemical tests). TPEs solve both the
sensitivity and cost issues and have been shown to be more robust than stencil printed carbon
electrodes (SPCE). The TPE surfaces will be covalently modified using electroreduction of
aryldiazonium salts followed by “click chemistry” to provide a platform for simple antibody
crosslinking reactions. The fully characterized and functional sensor will then be the basis for
future work integrating it into a microfluidic device for a full on-chip assay.

## Key facts

- **NIH application ID:** 10635462
- **Project number:** 3R01EB031510-01S1
- **Recipient organization:** COLORADO STATE UNIVERSITY
- **Principal Investigator:** DAVID S DANDY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $58,053
- **Award type:** 3
- **Project period:** 2021-09-13 → 2023-09-12

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10635462

## Citation

> US National Institutes of Health, RePORTER application 10635462, A SARS-CoV-2 NFC ePAD Biosensor (3R01EB031510-01S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10635462. Licensed CC0.

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