# Biogenesis of mRNA-derived telomerase long noncoding RNA

> **NIH NIH R01** · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · 2023 · $302,473

## Abstract

Project Summary
 Most RNA molecules transcribed in mammalian cells do not encode for protein sequences. Among these
noncoding RNAs (ncRNA) is a vast family of long noncoding RNAs (lncRNAs) that are larger than 200 nt.
LncRNAs can modulate cellular protein expression patterns by influencing the transcription of many genes, the
post-transcriptional fate of mRNAs and ncRNAs, and the turnover and localization of proteins. Telomerase
RNA is a unique class of lncRNA that functions as an integral component of the telomerase ribonucleoprotein
complex which maintains genomic stability and cellular immortality in cancer and stem cells. The overarching
goal of this project is to understand the mechanism and regulation of a novel mRNA-derived biogenesis of
telomerase RNA (lncRNA) in Ustilago maydis, a basidiomycete fungus.
 Telomerase RNA in Ascomycete yeasts and animals, telomerase RNAs are transcribed by RNA
polymerase II and share biogenesis pathways with small nuclear RNA (snRNA) and box H/ACA small
nucleolar RNA (snoRNA), respectively. In contrast, telomerase RNAs in ciliates and plants are transcribed by
RNA polymerase III. These distinct biogenesis mechanisms employed by these homologous telomerase RNA
molecules from different eukaryotic kingdoms or groups provide unparalleled opportunities for understanding
the fundamental principles underlying the biogenesis and evolution of the vast varieties of noncoding RNA
species in biology.
 We have recently identified the first Basidiomycete telomerase RNA from U. maydis, a fungal model
organism. Animal and yeast TRs are transcribed by RNA polymerase II with a protective cap at the 5’-end.
intriguingly, the U. maydis TR lacks a protective 5’ cap and is processed from the 3’-untranslated region of a
protein-coding mRNA precursor. In this research program, we will identify determinants in the TR precursor
that regulate the biogenesis of the mature U. maydis TR from the protein-coding mRNA. We will also study the
function and expression of the protein encoded in the mRNA precursor. Lastly, we will identify telomerase
accessory proteins in U. maydis and determine their roles in regulating the U. maydis TR biogenesis.
Successful outcomes of these specific aims will provide comprehensive and exciting details needed for
understanding the unprecedented mRNA-derived biogenesis mechanism of telomerase lncRNA.

## Key facts

- **NIH application ID:** 10638429
- **Project number:** 1R01GM149864-01
- **Recipient organization:** ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
- **Principal Investigator:** Julian J-L Chen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $302,473
- **Award type:** 1
- **Project period:** 2023-05-16 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10638429

## Citation

> US National Institutes of Health, RePORTER application 10638429, Biogenesis of mRNA-derived telomerase long noncoding RNA (1R01GM149864-01). Retrieved via AI Analytics 2026-05-29 from https://api.ai-analytics.org/grant/nih/10638429. Licensed CC0.

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