# Novel methods to improve nuclease mediated homologous recombination, Administrative suppl

> **NIH NIH R42** · ATGC, INC. · 2022 · $313,344

## Abstract

Project Abstract
In this Administrative Supplement grant application, we request funds to purchase a new cell
sorter (S3e™ Cell Sorter, Bio-Rad). This state of art cell sorter will enable us to evaluate large
size gene knock-in events, including both on-target knock-in, on-target insertion/deletion (indel)
rates as well as potential off-target knock-in and indel events, at the single cell resolution. In Aim
1, we plan to use this cell sorter to evaluate mi-spCas9-v2. In Aim 2, we will use this cell sorter
to evalute mi-saCas9. In Aim 3, we will use this cell sorter to evaluate the efficacy and safety
profiles of miCas9 in clinically relevant applications. As a results, this new cell sorter, once
equipped, will generate more convincing data to demonstrate the advantages of the proposed
miCas9 gene editing nucleases.

## Key facts

- **NIH application ID:** 10640401
- **Project number:** 3R42GM122181-02S1
- **Recipient organization:** ATGC, INC.
- **Principal Investigator:** Jifeng Zhang
- **Activity code:** R42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $313,344
- **Award type:** 3
- **Project period:** 2022-07-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10640401

## Citation

> US National Institutes of Health, RePORTER application 10640401, Novel methods to improve nuclease mediated homologous recombination, Administrative suppl (3R42GM122181-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10640401. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*