# 200keV, Energy Filtered, Intermediate-High Voltage Transmission Electron Microscope(IVEM)"

> **NIH NIH S10** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2023 · $2,000,000

## Abstract

PROJECT SUMMARY / ABSTRACT
This application outlines our request for funding to procure a new 200keV, energy filtered, intermediate-high
voltage transmission electron microscope (IVEM) (Thermo-Fisher/FEI Talos F200C G2). This instrument will be
completely unique to the La Jolla, CA research Mesa (and it’s many research organizations) and bring into
production use cutting edge (and complementary) methods for energy filter enabled, multiple label transmission
electron microscopy (e.g., ColorEM) and high throughput 3D electron microscopic tomography (3D EM) of semi-
thick epoxy-embedded samples of cells and tissues (non-Cryo). Procurement of this microscope will address
two pressing needs: 1) it will directly support growing demand for these imaging methods/technologies by both
local and Nationally distributed NIH investigators, currently users of the National Center for Microscopy and
Imaging Research (NCMIR); and 2) it will provide production access to cutting-edge methods previously only
available on high-end, specialized platforms built to enable development of methods like ColorEM, (fulfilling a
goal of NIH to lower barriers to the use and to promote broader dissemination and impact). As such, it will also
propel the democratization of these key technologies. The specified new instrument will be procured at a
significantly lower cost, compared to the instrument it will replace, a 20 year old custom-built JEOL 3200 IVEM,
soon not maintainable by JEOL. The new tool also simplifies installation, with more modest room requirements,
allowing adoption of the methods it enables by more NIH researchers. The platform will be automated and set-
up for easy access by the community and be supported by a team of experts in electron microscopy, specimen
preparation and data processing, analysis, and visualization. Its environment will also include (and make direct
use) of novel high-performance computing and data storage infrastructure of the San Diego Supercomputer
Center and its affiliated National Research Platform (NRP).

## Key facts

- **NIH application ID:** 10642585
- **Project number:** 1S10OD034447-01
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Mark H Ellisman
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $2,000,000
- **Award type:** 1
- **Project period:** 2023-09-10 → 2025-03-09

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10642585

## Citation

> US National Institutes of Health, RePORTER application 10642585, 200keV, Energy Filtered, Intermediate-High Voltage Transmission Electron Microscope(IVEM)" (1S10OD034447-01). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/10642585. Licensed CC0.

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