# Administrative Diversity Supplement to Genetics and Molecular Biology of Striated Muscle Myosin

> **NIH NIH R37** · SAN DIEGO STATE UNIVERSITY · 2022 · $51,793

## Abstract

ABSTRACT
This is a diversity supplement for Ph.D. candidate Jared Rafael Camillo to provide him guided career
development while working on his dissertation project, which is related to an NIH MERIT award to Sanford I.
Bernstein entitled Genetics and Molecular Biology of Striated Muscle Myosin. We will study two cardiac myosin
heavy chain isoforms that are expressed in Drosophila cardiomyocytes, in order to compare ATPase, motility
and structural properties. The latter will be determined using cryo-electron microscopy, which will allow
visualization of interactions with actin. As the two isoforms differentially affect muscle function when expressed
in indirect flight and jump muscles, we hypothesize that our studies will yield insights into cardiac myosin
structure/function relationships. We will then use CRISPR to introduce dilated cardiomyopathy (DCM) mutations
into each of the cardiac isoforms. We will assess the effects of the mutations on ATPase, motility and molecular
structure. We will then examine flight and jump muscle structure of mutant muscle via transmission electron
microscopy and function via flight and jump assays. The Swank lab (RPI) will measure impacts on force, velocity,
work, power generation, and identify steps of the myosin cross-bridge cycle that are altered. This integrative
analysis will test the hypothesis that DCM mutations yield reduced contractility. Overall, we will gain insights into
how the biochemical and structural properties of two cardiac myosin isoforms yield different functional properties
in muscle and how dilated cardiomyopathy mutations affect myosin and muscle structure and function.

## Key facts

- **NIH application ID:** 10645925
- **Project number:** 3R37GM032443-37S1
- **Recipient organization:** SAN DIEGO STATE UNIVERSITY
- **Principal Investigator:** Sanford I Bernstein
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $51,793
- **Award type:** 3
- **Project period:** 1983-07-01 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10645925

## Citation

> US National Institutes of Health, RePORTER application 10645925, Administrative Diversity Supplement to Genetics and Molecular Biology of Striated Muscle Myosin (3R37GM032443-37S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10645925. Licensed CC0.

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