# Characterization of the roles and regulation of Draxin in cranial neural crest

> **NIH NIH R00** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2023 · $249,000

## Abstract

PROJECT SUMMARY
 The neural crest (NC) is a stem cell population that originates within the forming central nervous
system. NC cells delaminate from the neuroepithelium by undergoing a spatiotemporally regulated epithelial-to-
mesenchymal transition (EMT) that proceeds in a coordinated wave head-to-tail to exit from the neural tube.
Cranial NC cells, which arise in the head region of the embryo and are the only NC population in vivo with the
ability to differentiate into craniofacial skeleton and cartilage, are indispensable for the development of the face;
mutations affecting NC development result in numerous diseases and malformations affecting the craniofacial
structures (e.g. Treacher Collins syndrome, cleft palate, etc.). Thus, a thorough understanding of the regulation
of cranial NC EMT is essential to identify the etiology of craniofacial abnormalities.
 To date, research has focused on unraveling early events governing cranial NC induction at the neural
plate border and the mechanism of delamination from the neural tube. However, little is known about the
regulatory changes governing the timing of NC EMT and exit from the neural tube. My preliminary studies
demonstrate that a Wnt pathway antagonist, Draxin, plays an important role in controlling the timing of NC
EMT, perturbations of which have negative consequences for cranial NC migration. This role for Draxin is
particularly interesting in light of the fact that the Wnt signaling pathway has been shown to be a major driver of
NC development. The goal of this Proposal is to investigate the molecular mechanism by which Draxin affects
cranial NC EMT, with focus on its role in Wnt signaling. To this end, the Specific Aims of this Proposal seek to
combine chick embryology with state-of-the-art imaging and biochemistry to:
 1) Characterize the interaction of Draxin and Wnt signaling in cranial NC EMT;
 2) Identify the role of Draxin in the regulation of cell adhesion proteins;
 3) Characterize the post-transcriptional regulation of Draxin; and
 4) Identify the role of Draxin in the regulation of extracellular matrix proteins.
 The results of this Proposal will significantly enhance our understanding of the regulation of cranial NC
EMT and migration, providing new scientific avenues for translational research applications in the treatment of
craniofacial defects.

## Key facts

- **NIH application ID:** 10653270
- **Project number:** 5R00DE028592-04
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Erica Hutchins
- **Activity code:** R00 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $249,000
- **Award type:** 5
- **Project period:** 2022-07-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10653270

## Citation

> US National Institutes of Health, RePORTER application 10653270, Characterization of the roles and regulation of Draxin in cranial neural crest (5R00DE028592-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10653270. Licensed CC0.

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