# Structural and Functional Characterization of RhoGEF Regulation Using Nanodiscs to Assemble Membrane-associated Signaling Scaffolds

> **NIH NIH R35** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2023 · $389,806

## Abstract

Project Summary/Abstract
Rho family small GTPases are key regulators of activities such as cell migration, gene transcription, growth, and
survival, processes initiated by signaling through diverse sets of molecules including cytokines, growth factors,
and GPCRs. Dbl family Rho guanine-nucleotide exchange factors (RhoGEFs), including around 70 members,
are critical activators of signaling by GTPases such as Rac, Cdc42, and Rho. RhoGEFs are multi-domain
proteins that frequently function as signaling scaffolds at cell membranes and play roles in regulating other
signaling pathways, a feature conferred by their complex architecture and the fact that each member has a
unique domain composition. As major players in processes underlying cell migration and division, several
RhoGEFs are strongly implicated in cancer. Despite their clinical importance, these proteins are vastly
understudied at the molecular level from a whole-molecule, mechanistic perspective, and there are no
therapeutic inhibitors that target these enzymes. Our laboratory is pioneering the study of full-length RhoGEFs
and mechanisms in their regulation at lipid membranes, using cryo-EM as a major approach. Our long-term goal
is to understand the complex, multi-component mechanisms behind Dbl RhoGEF signaling and regulation.
Within this family, the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (P-Rex)
subfamily, including P-Rex1 and P-Rex2, act as important regulators of cell migration. Both isoforms have been
associated with human cancers, wherein they act as pro-metastatic factors. P-Rex2 is commonly mutated in
breast cancer and melanoma, with mutations distributed throughout the protein. One study identified it as one of
the most mutated genes in human metastatic melanomas. Altogether, data support that P-Rex is an important
signaling molecule implicated in disease and a suitable therapeutic target. However, even though P-Rex was
discovered over 15 years ago, the molecular details of its regulatory mechanisms are still not fully understood.
P-Rex proteins are hypothesized to be autoinhibited in their inactive, non-signaling states and activated in multi-
step mechanisms. They are activated by binding membrane-tethered G protein b and g subunits, downstream of
GPCR signaling, and by binding cell membrane lipids, including the lipid PIP3. Additionally, P-Rex acts as a
signaling scaffold in various cellular contexts by binding to signaling proteins like PKA and PTEN, resulting in
changes in activities of P-Rex and the binding partner. Our long-term goal is to understand how different
RhoGEFs transition between the basal and fully active states and how this transition is regulated, starting with
the P-Rex subfamily. Furthermore, we will determine how these proteins act as signaling scaffolds at the cell
membrane. Using nanodiscs, we will study the multi-valent interactions of RhoGEFs with lipids and regulatory
molecules, giving us unprecedented insight into these...

## Key facts

- **NIH application ID:** 10662548
- **Project number:** 5R35GM146664-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** Jennifer Nicole Cash
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $389,806
- **Award type:** 5
- **Project period:** 2022-08-01 → 2027-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10662548

## Citation

> US National Institutes of Health, RePORTER application 10662548, Structural and Functional Characterization of RhoGEF Regulation Using Nanodiscs to Assemble Membrane-associated Signaling Scaffolds (5R35GM146664-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10662548. Licensed CC0.

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