# The ancestry of animal cell differentiation and pluripotency

> **NIH NIH R35** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2023 · $403,750

## Abstract

ABSTRACT
 Animals build their multicellular bodies with diverse types of cells that perform and integrate distinct
functions. Animals, however, are not unique in their capacity to generate distinct cell types. In fact, their closest
living relatives, a group of aquatic, unicellular, bacterivorous protists called choanoflagellates, detect biotic and
abiotic cues to differentiate into phenotypically and functionally distinct cell types in different environments.
Choanoflagellates also possess critical genes that regulate animal cell differentiation during development,
supporting the hypothesis that cell differentiation mechanisms evolved prior to the origin of animals and
became integral in animal and choanoflagellate biology.
 Although nearly 800 million years of animal evolution has shaped human biology since we last shared a
common ancestor with choanoflagellates, the commonalities in genetic toolkits and cytological characteristics
indicate that choanoflagellates have tremendous potential as microeukaryotic models to investigate the core
functions of genes that regulate cell differentiation. During my postdoc, I pioneered the first methods for gene
delivery and genome editing in the choanoflagellate Salpingoeca rosetta to realize its full potential as a model
system. My lab continues to propel those methods for the discovery of the molecular mechanisms that drive
environmentally-triggered cell differentiation in S. rosetta. This proposal supports our research mission by using
the molecular tools I developed to dissect putative regulatory pathways that emerge from functional genomic
surveys. In particular, we focus on homologs of RNA-binding proteins that form the animal germline and and/or
maintain pluripotency. Moreover, we strive to develop our nascent genetic tools into scalable, easy methods that
enable genome-wide screens of cell differentiation regulators. Overall, this work will contribute a new, functional
comparison to illuminate the origin and evolution of cell differentiation pathways in choanoflagellates and
animals, which I anticipate will uncover core functions of biomedically important genes that originated before
choanoflagellates and animals diverged.

## Key facts

- **NIH application ID:** 10669792
- **Project number:** 5R35GM147404-02
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** David Scott Booth
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $403,750
- **Award type:** 5
- **Project period:** 2022-08-01 → 2027-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10669792

## Citation

> US National Institutes of Health, RePORTER application 10669792, The ancestry of animal cell differentiation and pluripotency (5R35GM147404-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10669792. Licensed CC0.

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