# Supplement to TR01 Human cortical development and neural plasticity altered by trisomy 21

> **NIH NIH R01** · UNIVERSITY OF WISCONSIN-MADISON · 2022 · $186,170

## Abstract

ABSTRACT
Down syndrome (DS, trisomy 21, T21), a complex multigene disorder and the most common genetic cause of
intellectual disability. However, surprisingly little is known about the underlying mechanisms that lead to cognitive
impairment in DS. There are fewer neurons in adult DS cortex and reduced neurogenesis and synaptogenesis
have been implicated as features of DS development. Yet, what and how specific neurons and synaptic contacts
are affected at which period of development and what molecular pathways underlie these defects that lead to
intellectual disability remain unclear. The parent award was funded to build models based on human induced
pluripotent stem cells (iPSCs), to interrogate how T21 disrupts developmental processes in DS. The iPSC model
will be validated by integrating cellular changes and molecular signatures of single cells in vitro with DS prenatal
cortex. The parent award relies on maintenance, differentiation and analysis of iPSC derived neurons in vitro for
two of the three Aims. This supplement requests funding for cell culture related equipment that is required to
carry out Aims 2 and 3 of the parent award. Funding is requested to replace two cell culture incubators for
maintenance of iPSCs and differentiated neurons in Aim 2 and 3. Funding is also requested for an additional
electrophysiological rig for analysis of iPSC derived neurons in Aim 3. The parent award included minimal
equipment funding and these costs were unforeseen when the application was awarded.

## Key facts

- **NIH application ID:** 10670626
- **Project number:** 3R01HD106197-01S1
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** ANITA BHATTACHARYYA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $186,170
- **Award type:** 3
- **Project period:** 2022-09-12 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10670626

## Citation

> US National Institutes of Health, RePORTER application 10670626, Supplement to TR01 Human cortical development and neural plasticity altered by trisomy 21 (3R01HD106197-01S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10670626. Licensed CC0.

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