# Flow Cytometry Shared Resources

> **NIH NIH P30** · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · 2023 · $225,944

## Abstract

PROJECT SUMMARY: FLOW CYTOMETRY SHARED RESOURCE
The Flow Cytometry Shared Resource (FCSR) at Sylvester Comprehensive Cancer Center (Sylvester) is a
state-of-the-art facility offering comprehensive analytical flow cytometry and high-speed cell sorting services
that are pivotal to the successful progress of many research projects. The FCSR Director and staff work
collectively to provide professional consultation, assay design, and research training as well as full cytometric
analysis and cell sorting services to support research programs, publications, and grant applications. The
FCSR’s six sophisticated fluorescence flow cytometry instruments (three cell analyzers and three cell sorters)
facilitate a wide range of complex experiments examining the biological properties of both normal and cancer
cells. The FCSR has the capability to provide simultaneous analysis of up to 19 different fluorescent
parameters and two laser light-scatter parameters (forward and side scatter) for both cell surface and
intracellular antigen detection in complex cell mixtures. The FCSR performs a variety of experimental analyses,
such as complex immunophenotyping, DNA content and cell cycle analysis, cell signaling and activation
analysis, and measurement of proliferation and apoptosis. Further, the FCSR provides fluorescence-activated
cell sorting services, which allow investigators to purify both bulk and single cells at high purity and high speed,
based upon complex multiparametric phenotyping. The three cell sorters have the potential to flow-sort up to
17 fluorescence parameters into bulk tubes or into multi-well plates for single cell flow cloning under Biosafety
Level 2 conditions. The FCSR’s three highly experienced technicians perform cell sorting as well as continuous
quality control assessment for all instruments. They also provide comprehensive user training in all aspects of
flow cytometric experiments, including experimental design, fluorescent reagent panel selection, instrument
setup and operation, data acquisition, and data analysis. Users are trained such that they are capable of
operating the analysis cytometers themselves and then are permitted to do so independently during or outside
regular operating hours. Users access the facility through an online instrument reservation and invoicing
system. In 2017, the FCSR provided cytometry services to 41 Sylvester members (95% of whom were
supported by peer-reviewed funding), which comprised 78% of total FCSR usage.

## Key facts

- **NIH application ID:** 10670841
- **Project number:** 5P30CA240139-05
- **Recipient organization:** UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
- **Principal Investigator:** Huw S. Kruger Gray
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $225,944
- **Award type:** 5
- **Project period:** 2019-07-10 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10670841

## Citation

> US National Institutes of Health, RePORTER application 10670841, Flow Cytometry Shared Resources (5P30CA240139-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10670841. Licensed CC0.

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