SUMMARY/ABSTRACT – Biospecimen and Pathology Core (BiPC) The Biospecimen and Pathology Core (Core 1), a biorepository and multi-faceted research resource, provides critical support for the translational and diagnostic/therapeutic studies of the three major SPORE projects, the Developmental Research projects and Career Enhancement programs of the UCLA SPORE in Brain Cancer. The SPORE projects and programs have a critical need for high-quality brain tumor biospecimens, associated clinical and molecular annotations, histology, immunohistochemistry, genomic sequencing, and characterization of models systems derived from the human biospecimens. The projects also require neuropathology and biobanking expertise to accomplish their research aims. For Aim 1, the core will optimally collect, store, prioritize and distribute high-quality brain tumor biospecimens. The core provides detailed annotation of collection and preservation of biospecimens and tracks them with pertinent data through interfacing with the data management platform of the Administrative Core (Core A) using Daedealus-BTM. Aim 2 of the core is to molecularly characterize and analyze SPORE patient-derived tumor tissue and derivative models specific for each SPORE project, using histologic analysis, immunohistochemistry, and genomic tools. The core provides a variety of neuropathology services related to the analysis of patient specimens (e.g. in situ hybridization, tissue microdissection, immunohistochemistry, genomic analysis), as well as services related to the pre-clinical studies and clinical trial components of the SPORE. The core also works with Core 3, the Biostatistics and Bioinformatics Core (BBC) to perform integrated comparative analyses of next generation molecular characterization of samples and models derived from human glioma patients (e.g., germline, whole tumor, purified tumor cells, gliomaspheres, and xenografts). Aim 3 of the core is to centrally create and provide novel human biospecimen derivatives, patient-derived orthotopic xenografts (PDOX), and organoids to support SPORE major and developmental projects. We will use our established protocols to create and characterize patient-derived gliomaspheres, adherent cultures, and direct-from-patient animal orthotopic xenograft models representing the molecular diversity of glioblastoma. Our novel gaussia (sGluc-GFP) reporter system will facilitate the analysis of xenografted tumor models. The core will provide and utilize cerebral organoids and other cellular derivatives to propagate rare tumor models and to study the tumor microenvironment, invasion, and therapeutic targets. The core will also collaborate with the Administrative Core (Core A), the Neuro-Imaging Core (NIC, Core 2) and the Biostatistics & Bioinformatics (BBC, Core 3) to develop an integrated clinical-radiologic-molecular pathological characterization database and provide this to SPORE investigators, SPORE-associated clinical trials (projects 1-3), and public ...