Project Summary Legionella pneumophila (Lp) is the agent of Legionnaires' disease, an oft-fatal pneumonia that is increasing in incidence. Lp is ubiquitous in water systems, infecting the lungs after inhalation of contaminated droplets. In waters, Lp persists as an intracellular parasite of amoebae and constituent of biofilms. In the lungs, it grows in macrophages, mimicking what it does in amoebae. Learning how Lp survives in water and grows in amoebae and biofilms is critical to understanding and possibly preventing human disease. In past, the Cianciotto lab showed that Lp encodes a type II secretion system (T2SS) which mediates secretion of >25 protein substrates. From mutant analysis, the T2SS was required for infection of at least 4 types of amoebae, macrophages, and the murine lung. The lab also identified 8 substrates that are required for infection of Acanthamoeba castellanii and other amoebae. Yet, given the large defect shown by mutants lacking the entire T2SS vs the more modest defects of mutants lacking individual substrates, I posited there are more important T2SS substrates to be found. When the lab had used proteomics to define the first 25 substrates, in silico analysis suggested that there are ~60 more substrates, and recent proteomics on Lp supernatants showed that 47/60 of the predicted substrates are in fact secreted. Since there had been a positive correlation between a T2SS substrate’s prevalence across the Legionella genus and the requirement of that substrate for infection of amoebae, I made mutants lacking each of the 9/47 “new” substrates that occur in > 93% of Legionella species. I then determined that the new substrate Lpw20501 majorly promotes infection of A. castellanii. After immunoblot confirmation of the T2SS-dependency of Lpw20501, bioinformatics revealed that the protein represents an uncharacterized family of polysaccharide deacetylases that is predicted to act on i) N-acetylglucosamine-containing compounds, which may include chitin, ii) acetyl-containing xylan or cellulose acetate, or iii) other (novel) acetyl- containing substrates. I further observed that the lpw20501 mutant aggregated more rapidly than WT did, suggesting that secreted Lpw20501 may uniquely deacetylate the outer surface of Lp and as a result impact biofilm formation. Thus, I posit that Lpw20501 is a novel secreted protein that enhances the survival of Lp in multiple intra- and extracellular niches. This proposal will i) purify Lpw20501 and discern its enzyme activity, ii) further define surface traits tied to Lpw20501, and iii) judge Lpw20501’s impact on Lp growth in various amoebae, in biofilms, and on acetyl-containing polysaccharides. This work will i) increase our knowledge of a key pathogen, ii) define a new type of exoenzyme, iii) have implications for other pathogens that use T2SS or are intracellular parasites, and iv) possibly define a new target for controlling Lp in the built environment.