# Novel Sensor Integrated Proteome on Chip (SPOC) platform for evaluating kinetic parameters of protein interactions in high throughput

> **NIH NIH R44** · SPOC PROTEOMICS INC. · 2023 · $1,070,227

## Abstract

Abstract
A critical bottleneck in the field of proteomics is the inability to simultaneously and cost-effectively
evaluate thousands of functional protein interactions at once to obtain kinetic data. Although
conventional in-situ protein microarray technology is well developed, it uses fluorescent dye
based detection that provides only qualitative or semi-quantitative information. There is currently
no technology that offers label-free real-time interaction assays of full-length folded proteins in
high throughput format. To address this unmet need, INanoBio is developing novel sensor
integrated proteome on chip (SPOC) platform that combines in situ expression and capture of
functional proteins on biosensors, to allow instant high throughput analysis of thousands of
proteins in real time. In this SBIR phase II project, we propose to develop SPOC platform by
integrating in situ produced protein arrays with surface plasmon resonance imaging (SPRi), by
building integrated ProSPR instrument that will enable SPRi determination of both qualitative and
kinetic information of protein biomolecular interactions in high throughput. We will develop,
validate and apply 1000 protein SPOC SPRi sensor chips to study clinically relevant protein-
protein interactions and test off-target binding of new antibodies produced in animal models
against human proteins.

## Key facts

- **NIH application ID:** 10683348
- **Project number:** 5R44TR004297-02
- **Recipient organization:** SPOC PROTEOMICS INC.
- **Principal Investigator:** Bharath Takulapalli
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $1,070,227
- **Award type:** 5
- **Project period:** 2022-08-12 → 2025-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10683348

## Citation

> US National Institutes of Health, RePORTER application 10683348, Novel Sensor Integrated Proteome on Chip (SPOC) platform for evaluating kinetic parameters of protein interactions in high throughput (5R44TR004297-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10683348. Licensed CC0.

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