# Calcium signaling controls Pseudomonas aeruginosa invasion and adaptation to the host intracellular environment

> **NIH NIH R15** · OKLAHOMA STATE UNIVERSITY STILLWATER · 2022 · $99,512

## Abstract

ABSTRACT
The currently funded research 2R15GM124670-02 is focused on discovering the role of Ca2+
signaling in the interactions of P. aeruginosa with host cells. These studies include establishing
the signaling patterns of Ca2+ homeostasis by monitoring the changes of Ca2+ levels in P.
aeruginosa during interactions with host cells, including invasion, intracellular survival, and
escape. To enable the accurate measurements of the temporal and spatial changes of Ca2+
concentration within subcellular spaces of P. aeruginosa cells, we request to replace the currently
obsolete plate reader Biotek with the updated version of the instrument, Cytation 5. The new
instrument offers enhanced capabilities and accuracy of measurements. It allows low-noise
reading of monochromator-based absorbance, tunable fluorescence, and luminescence.
Importantly, it will also include imaging using 20X – 60X objectives, high contrast brightfield and
four different imaging cubes for fluorescence. It allows tight temperature control with a 4-zone
temperature control system and prevents condensation on plate lids. It offers dual reagent
injectors able for multimode detection. The requested instrument will be placed in the PI's lab and
will be available for the use by all the students in the PI's lab and the collaborating lab of Dr.
Lutter. All the participating students will be trained to use the instrument and the accompanying
software by the company representative. The anticipated research advances will significantly
enhance our ability to study calcium signaling in P. aeruginosa interacting with its eukaryotic host.
The obtained new knowledge will improve our understanding of this important human pathogen
and inform the development of more efficient means for controlling or preventing its deadly
infections.

## Key facts

- **NIH application ID:** 10685112
- **Project number:** 3R15GM124670-02S1
- **Recipient organization:** OKLAHOMA STATE UNIVERSITY STILLWATER
- **Principal Investigator:** Marianna M. A. Patrauchan
- **Activity code:** R15 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $99,512
- **Award type:** 3
- **Project period:** 2017-08-01 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10685112

## Citation

> US National Institutes of Health, RePORTER application 10685112, Calcium signaling controls Pseudomonas aeruginosa invasion and adaptation to the host intracellular environment (3R15GM124670-02S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10685112. Licensed CC0.

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