# Flow Cytometry

> **NIH NIH P30** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2023 · $212,310

## Abstract

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE 
The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- 
speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell 
sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical 
cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then 
independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained 
users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX 
MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have 
autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional 
site license. This broadly used Core is managed by the CC and receives support from the Institute for 
instrumentation purchase and maintenance. In the last funding period, three major technology platforms were 
acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff 
have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and 
they provide comprehensive technical guidance at any stage of a project, from experimental design through 
manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 
Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related 
publications.

## Key facts

- **NIH application ID:** 10686131
- **Project number:** 5P30CA030199-42
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** Aniruddha J. Deshpande
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $212,310
- **Award type:** 5
- **Project period:** 1997-05-01 → 2025-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10686131

## Citation

> US National Institutes of Health, RePORTER application 10686131, Flow Cytometry (5P30CA030199-42). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10686131. Licensed CC0.

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