# Cancer Metabolism

> **NIH NIH P30** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2023 · $191,201

## Abstract

ABSTRACT – CANCER METABOLISM SHARED RESOURCE 
The Cancer Metabolism Shared Resource provides comprehensive support for analysis of cancer-related 
metabolism by investigators at the Sanford Burnham Prebys Medical Discovery Institute NCI-supported Cancer 
Center. The services provided include assistance with the design of metabolic research programs and selection 
of appropriate analytical methods, sample analysis using specialized equipment and methods, assistance with 
data interpretation and publication (including response to reviewers), and assistance with grant proposals. Dr. 
Scott, the facility Director, has extensive experience in metabolic analysis and serves as a resource for Center 
members, educating scientists about the services offered, working to upgrade existing methods, and expanding 
the range of analyses provided, both in response to requests from users and proactively in anticipation of future 
demand. The Core provides three fundamental types of analysis. The first is quantitation of metabolites, either 
through GC-MS analysis or a 96-well YSI analyzer. In the past funding period (the first for this Shared Resource), 
a variety of enhancements have been made in quantification of metabolites such as fatty acids (including short- 
chain fatty acids), cholesterol, sugars, and sugar phosphates, as well as more standard detection of glucose, 
lactate, glutamine, and glutamate. The second approach provided in the Core is stable isotope tracing, 
where(13C, 15N, 2H)-labeled substrates can be traced to metabolites such as TCA cycle intermediates and fatty 
acids, which provides information on metabolic pathway flux that provides insight beyond metabolite 
quantification alone. The third major analytical platform in the Core is a Seahorse XFp Extracellular Flux 
Analyzer, purchased in 2015, which allows real-time measurement of cellular oxygen consumption and 
extracellular acidification. Mitochondrial function and the relative contribution of glycolysis and oxidative 
phosphorylation to ATP production can be measured in live by sequential addition of various mitochondrial 
inhibitors or uncouplers. Over the last 5 years, the Cancer Metabolism Core has provided services to 24 Cancer 
Center members, and supported at least 20 publications

## Key facts

- **NIH application ID:** 10686153
- **Project number:** 5P30CA030199-42
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** Jorge Moscat
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $191,201
- **Award type:** 5
- **Project period:** 1997-05-01 → 2025-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10686153

## Citation

> US National Institutes of Health, RePORTER application 10686153, Cancer Metabolism (5P30CA030199-42). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10686153. Licensed CC0.

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