# Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)

> **NIH NIH U24** · STANFORD UNIVERSITY · 2023 · $695,930

## Abstract

In response to the NIH RFA-RM-19-009, we propose to establish the Stanford-SLAC CryoET Specimen
Preparation Service Center (SCSC) to accomplish four specific aims: (1) establish a platform, which is available
to the scientific community at large, to streamline the preparation of samples suitable for downstream cryogenic
electron tomography (cryoET) data collection; (2) provide access to advanced cryo-specimen preparation
techniques for a wide range of samples: macromolecular complexes, microcrystals of biological materials, cell
lysates, organelles, cells and tissues; (3) generate a training curriculum for new users on the preparation of
frozen, hydrated biological specimens, with the option of carrying out correlative cryo-fluorescence light
microscopy (cryoFLM), cryo-focused ion beam scanning electron microscopy (cryoFIB), and cryoET; and (4)
adopt new methods for sample preparation using innovative technologies developed elsewhere. In this proposal,
we will leverage our existing facilities and in-house expertise in each of the following imaging modalities and their
integration into a correlated sample preparation workflow: cryoFLM, cryoFIB, and cryoET. We will establish all
the necessary equipment dedicated to the proposed service and training activities. We will prepare video
curriculum materials to train new users in the aforementioned protocols. We will adapt our existing infrastructure
to manage project administration and resource allocations. Administrative support is in place to help users with
lodging arrangements, as well as laboratory on-boarding and specimen biosafety approvals. We have a well set-
up communication infrastructure for remote users to participate in actual experiments, particularly for cryoFLM,
cryoFIB, and cryoET. We will provide access to existing Talos Arctica and/or Titan Krios microscopes to evaluate
whether the prepared samples are ready for data collection in the associated Hub, which will be set up at another
institution with separate NIH support. We will make our existing Stanford-SLAC cryo-specimen preparation
equipment available on a limited basis immediately after this proposal is funded. We anticipate serving ~40 users
per year once the Center is fully operational in early Year 2 of the award period. We will also offer regular hands-
on workshops to train ~10-12 new users per year. We will disseminate our resources to the broad community
via a web portal and booths at professional societies’ annual conferences. The number of users will increase in
subsequent years as we and our recurring users optimize the use of the Center’s resources. In Years 3-6, we
will work closely with collaborators pioneering advanced protocols such as semi-automated and automated
cryoFIB and “lift-out” for tissue samples to implement them in the proposed Center. Lastly, we will assemble a
Scientific Advisory Committee of experts to guide the practices of our Service Center and the ongoing
implementation of cutting-edge technologie...

## Key facts

- **NIH application ID:** 10691374
- **Project number:** 5U24GM139166-04
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** Wah Chiu
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $695,930
- **Award type:** 5
- **Project period:** 2020-09-16 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10691374

## Citation

> US National Institutes of Health, RePORTER application 10691374, Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) (5U24GM139166-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10691374. Licensed CC0.

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