# Defining the intestinal stem cell niche during organoid development into a functional human intestine

> **NIH NIH U01** · CINCINNATI CHILDRENS HOSP MED CTR · 2022 · $30,175

## Abstract

Abstract
This proposal capitalizes on our recently developed in vitro methods to culture human pluripotent stem cell
derived intestinal and colonic organoids (HIO/HCO), combined with our unique organoid transplantation
models to identify specific mesenchymal and endodermal niche cell types that support the small and large
intestine. The clinical relevance of this work done within the Intestinal Stem Cell Consortium (ISCC) is that it
will contribute to our groups effort to identify optimal ISC niche components that will enable both
transplantation of functional human regionalized intestine and inform therapies required to regenerate damage
intestine. Our preliminary data demonstrates the successful development of reporter tools and recombination
assays to localize specific mesenchymal and endodermal populations with cutting edge molecular approaches
to identify specific signal pathways supporting the human ISC and in vivo human intestinal models to study
regeneration following systemic administration of chemotherapy.

## Key facts

- **NIH application ID:** 10694310
- **Project number:** 3U01DK103117-09S1
- **Recipient organization:** CINCINNATI CHILDRENS HOSP MED CTR
- **Principal Investigator:** MICHAEL A. HELMRATH
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $30,175
- **Award type:** 3
- **Project period:** 2014-09-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10694310

## Citation

> US National Institutes of Health, RePORTER application 10694310, Defining the intestinal stem cell niche during organoid development into a functional human intestine (3U01DK103117-09S1). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10694310. Licensed CC0.

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