# Elucidating Electrical Stimulation Induced Non-Neuronal Activity Using Emerging in Vivo Imaging Technology and Electrophysiology

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2022 · $7,075

## Abstract

Project Summary
 Electrical microstimulation has become a mainstay of fundamental neuroscience exploration and an
increasingly prevalent clinical therapy. Despite the growing prevalence of neuromodulation therapies, the
fundamental physiological and mechanistic properties driving the beneficial effect for the patient are poorly
understood. This R01 application aims to greatly improve our understanding of how different non-neuronal
cells (myeloid lineage, oligodendrocyte progenitor lineage, and vascular smooth muscle cells) respond and
contribute to the electrical stimulation response. Understanding of the relationship between stimulation
parameters and supporting non-neuronal cell activity, including blood flow, will help determine the impact of
electrical microstimulation on chronic circuit behavior in-vivo over time. In this proposal, we use leading-edge in
vivo multiphoton imaging techniques with multiple transgenic animals to systematically evaluate the
relationship between stimulation parameters and the induced changes over time at the molecular, cellular, and
local network. An improved understanding of the impact of electrical microstimulation on the overall tissue
health, changes to the foreign body response, stimulation of tissue repair, and safety limits will help inform
improved stimulation paradigms and device design for therapeutic applications and basic neuroscience
research.

## Key facts

- **NIH application ID:** 10694329
- **Project number:** 3R01NS115707-03S1
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Takashi Daniel Yoshida Kozai
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $7,075
- **Award type:** 3
- **Project period:** 2020-09-30 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10694329

## Citation

> US National Institutes of Health, RePORTER application 10694329, Elucidating Electrical Stimulation Induced Non-Neuronal Activity Using Emerging in Vivo Imaging Technology and Electrophysiology (3R01NS115707-03S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10694329. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*