# The Cell Phenotyping and Mouse Core

> **NIH NIH P01** · NORTHWESTERN UNIVERSITY · 2023 · $366,466

## Abstract

Project Summary
The Cell Phenotyping and Mouse Core, designated as Core C, is a centralized facility that will provide
investigators with a well-established and reproducible model of influenza A–induced pneumonia. Core C will
generate stocks of influenza (A/WSN/33) virus, titrate and maintain viral stocks, and perform infection of the
experimental animals as outlined in Projects 1, 2, 3 and 4. Quantitative tools such as Flexivent measurements
of lung mechanics, quantitative histological assessment, flow cytometric phenotyping and sorting of the
immune cellular populations, and multiplex measures of pro-inflammatory cytokines will be conducted by Core
C according to the research plan outlined in Projects 1, 2, 3 and 4. Core C will use advanced flow cytometry
capabilities to quantify, phenotype, and sort specific inflammatory and parenchymal cell populations (such as
tissue-resident and recruited alveolar macrophages, perivascular and peribronchial interstitial macrophages,
monocyte subsets, dendritic cell subsets, T, B and NK cell subsets, alveolar epithelial type II cells, fibroblast
subsets, endothelial cells) from the murine lung and lymphoid organs. The sorting and isolation capabilities of
the Core will allow cell-type-specific assessment of transcriptomic response via RNA-sequencing, including
single-cell RNA-sequencing. To this matter, Core will provide automated DNA/RNA isolation from sorted cells,
followed by quality assessment and RNA-sequencing library construction and sequencing. Core C will breed
mice to generate the cell-type- or tissue-specific Cre recombinase lines to induce tissue-specific knockout
mice. Core C will perform the genotyping of all the murine strains proposed by Project Leaders. Core C will
maintain a uniform environment in which wild-type and genetically engineered mice will be maintained,
subjected to lung injury and allowed to recover. Collectively, these tools provide a unique resource for Project
Investigators, which would be difficult to reproduce without the support of this PPG.

## Key facts

- **NIH application ID:** 10696959
- **Project number:** 5P01HL154998-03
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** Bria M Coates
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $366,466
- **Award type:** 5
- **Project period:** 2021-09-15 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10696959

## Citation

> US National Institutes of Health, RePORTER application 10696959, The Cell Phenotyping and Mouse Core (5P01HL154998-03). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10696959. Licensed CC0.

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