# Mitochondrial Imaging, PTM Analysis and Metabolism Core

> **NIH NIH P01** · FLORIDA INTERNATIONAL UNIVERSITY · 2023 · $331,695

## Abstract

ABSTRACT
The goal of the Mitochondrial Imaging, PTM analysis, and Metabolism Core (Core B) is to provide a high-
quality state-of-the art service to support analyses in all three of the PPG Projects. In this context, Core B will
be defined by providing four services. 1) High resolution imaging of mitochondria. This will include
analyses of specific protein redistribution to mitochondria, mitochondrial network remodeling, and markers
of mitochondrial function (mitochondrial derived reactive oxygen species, mitochondrial membrane
potential, carnitine metabolites, ATP levels). 2) Bioenergetics: oxygen consumption, rate of glycolysis, fatty
acid oxidation, measurement of cellular ATP kinetics, ETC complex analyses in mechanical stress
challenged cells. 3) The analysis of PTMs in biological samples. Individual proteins will be purified to assay
PTMs in vivo. To analyze PTMs, we will utilize a mass spectrometry approach coupled to the detection and
quantification of specific sites of modification by tandem mass spectrometry. 4) Metabolomic analyses of
individual metabolites in cultured cells, lung tissue, and plasma from animals and children born with
congenital heart disease that results in changes in pulmonary blood flow (PBF). Core B will enhance the
scientific work for all three projects by assuring that all mitochondrial, PTM, and metabolomic analyses
are standardized and performed in a uniform standardized manner, and by highly experienced and
trained personnel. Core B is run by experienced investigators who have had many years of experience
using these techniques. They have developed or adapted many of these techniques and applied them to
perform physiological studies in in vitro and in vivo systems. As a central dedicated analytical resource Core
B will ensure these analyses are carried out in a cost-efficient manner.

## Key facts

- **NIH application ID:** 10705684
- **Project number:** 5P01HL146369-05
- **Recipient organization:** FLORIDA INTERNATIONAL UNIVERSITY
- **Principal Investigator:** Stephen M Black
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $331,695
- **Award type:** 5
- **Project period:** 2020-08-20 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10705684

## Citation

> US National Institutes of Health, RePORTER application 10705684, Mitochondrial Imaging, PTM Analysis and Metabolism Core (5P01HL146369-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10705684. Licensed CC0.

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