# Core B: Protein Expression Core

> **NIH NIH P01** · CALIFORNIA INSTITUTE OF TECHNOLOGY · 2023 · $411,538

## Abstract

Biochemical, structural, and immunization experiments to evaluate new immunogens designed 
and selected to induce broadly neutralizing antibodies will require expression and purification of 
large numbers of proteins, including HIV envelope proteins and antibodies. The Protein 
Expression Core will provide purified proteins for this program project, which will facilitate the 
goals of identifying immunogens and defining immunization strategies that elicit a strong and 
targeted immune response. Antibodies isolated from immunized mice (Project 1) will be 
expressed and purified for binding and biochemical characterization as well as for structural 
studies (Project 2). Env proteins, both monomeric gp120s and native-like Env gp140 trimers 
(SOSIP.664 constructs), will be expressed for immunizations in Project 1, binding studies in 
Projects 1 and 2, and structural/biochemical analyses in Project 2. Core B will use various 
expression systems to produce Env proteins with different glycosylation to determine the effects 
of glycosylation form on the generated immune response. The large number of different 
antibodies and HIV proteins to be expressed and purified for this program requires a dedicated 
protein expression core.

## Key facts

- **NIH application ID:** 10710277
- **Project number:** 5P01AI100148-10
- **Recipient organization:** CALIFORNIA INSTITUTE OF TECHNOLOGY
- **Principal Investigator:** Pamela J Bjorkman
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $411,538
- **Award type:** 5
- **Project period:** 2013-02-10 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10710277

## Citation

> US National Institutes of Health, RePORTER application 10710277, Core B: Protein Expression Core (5P01AI100148-10). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/10710277. Licensed CC0.

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