Project Summary/Abstract The UCSF Brain Tumor SPORE Biospecimen/Pathology Core provides staff and technology dedicated to the procurement, processing, storing, distribution, histopathologic, and molecular analyses of high-quality brain tumor biospecimens for translational science research. Our mission is to enhance biospecimen quality and utility through use of optimized standard operating procedures, multi-modality preservation, integrated histopathologic and molecular annotation, and a computerized inventory, request and tracking system. All aspects of this Core are performed in accordance with the guiding principles set forth in the 2016 National Cancer Institute Best Practice for Biorepository Guidelines. In order to maximize sharing and integration of SPORE projects, the Biospecimen Core collects and makes available data derived from all distributed brain tumor biospecimens. Specific Aims of SPORE Biospecimen/Pathology Core: 1. To acquire and preserve high-quality brain tumor patient biospecimens from the operating room and from preclinical models used by the Projects with optimized handling to maximize cell viability and/or minimize the cold-ischemia time, so as to meet the tissue accrual requirements for all of the proposed Brain Tumor SPORE projects and clinical trials. 2. To provide standardized routine and advanced tissue handling/processing and analytical techniques, including multiplex immunoflourescence, multiplexed protein and RNA assays, tissue microarray construction, quantitative whole slide image analysis, DNA/RNA extraction, and preparation of viable cells that will allow each SPORE project to fulfill its goals. 3. To provide biospecimen and neuropathology expertise to ensure optimal biospecimen quality and extraction of high-quality data from biospecimens collected from the operating room and animal models. This includes performing quality control assays on archived biospecimens and extraction of appropriate quantitative and semi-quantitative parameters from whole slide images. 4. To provide consistent oversight and tracking of the Core activities through the SPORE Biospecimen/Pathology Core database. This database will contain demographic data, integrated histopathologic and molecular annotation, results from molecular analyses, and tissue distributions (internal and external) that will be linked to relational clinical databases maintained by the SPORE Biostatistics and Clinical Core and used by all SPORE Projects. In addition, biospecimen tracking in the database will ensure stringent compliance with regulations governing informed consent and patient confidentiality.