# Resource for Multiphoton Characterization of Genetically-Encoded Probes

> **NIH NIH U24** · MONTANA STATE UNIVERSITY - BOZEMAN · 2024 · $360,197

## Abstract

Project Summary/Abstract
Two-photon laser scanning microscopy powers many projects in the BRAIN initiative because
it is the best way to capture the concerted activity of living neural networks. Genetically-
encoded activity probes and sensors built with fluorescent proteins are the tools of choice for
labeling and recording from living circuits of neurons. However, to date, virtually all
optimization of these probes has been solely in the one-photon regime. This is mainly because
measuring quantitative two-photon absorption properties demands more sophisticated
equipment and expertise in photophysics and nonlinear optics. The BRAIN initiative has driven
many laboratories to create new genetically encoded fluorescent probes and biosensors. To
characterize their multiphoton properties we created a special Resource for Multiphoton
Characterization of Genetically-Encoded Probes that is supported by NIH/NINDS. This
characterization is indispensable because it identifies optimum wavelengths for 2P excitation,
and aids quantitative comparison of 2P brightness and 2P dynamic range (for biosensors).
Over the previous funding period our Resource was used by 17 different investigators and if
the grant renewed, this number will increase to 30 labs by the end of the 5th year of the next
cycle.
 As in the previous funding period, we will provide a very broad access to our facility for
Investigators whose research is relevant to the NIH/NINDS mission and to the goals of the
BRAIN initiative. The information obtained will be broadly disseminated via scientific
publications and two community-driven websites. We will organize annual workshops where
we will provide training on 2PA photophysics and methods of measurements and provide all
necessary information for researchers who were willing to start such measurements in their
labs.

## Key facts

- **NIH application ID:** 10724471
- **Project number:** 2U24NS109107-06
- **Recipient organization:** MONTANA STATE UNIVERSITY - BOZEMAN
- **Principal Investigator:** Mikhail Drobizhev
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $360,197
- **Award type:** 2
- **Project period:** 2018-09-30 → 2029-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10724471

## Citation

> US National Institutes of Health, RePORTER application 10724471, Resource for Multiphoton Characterization of Genetically-Encoded Probes (2U24NS109107-06). Retrieved via AI Analytics 2026-06-14 from https://api.ai-analytics.org/grant/nih/10724471. Licensed CC0.

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