# Project 2: Immune Response Analysis

> **NIH NIH P01** · SCRIPPS RESEARCH INSTITUTE, THE · 2023 · $510,527

## Abstract

PROJECT SUMMARY
The purpose of this Project is to apply advanced multi-omics techniques to comprehensively analyze humoral
immune responses to promising HIV vaccine immunogens. Our mission is to advance vaccine development by
extracting as much information as possible from each immunization trial to best inform the optimal design,
composition and delivery of a cohesive immunogen matrix that reliably induces broad, protective HIV immunity.
We have devised three Specific Aims which outline the steps necessary to accomplish these goals. Successful
completion of these Aims will require indispensable contributions from every component of the MOVE
Consortium. In Aim 1, we will deeply characterize the immune response to priming immunogens designed to
activate bnAb precursors of one or more specificities (Project 1). While many aspects of this Aim are designed
to evaluate the extent to which immunogens are working as designed, equally important is identifying specific
genetic and structural features which need improvement during subsequent rounds of refinement and re-
evaluation (Structural Proteomics Core). In Aim 2, we will evaluate the human immunogenicity of candidate
priming immunogens by isolating immunogen-specific mAbs from HIV-naive humans. This is a critically
important aspect of our vaccine development process, as these mAbs are most accurate representation, short
of a human clinical trial, of the humoral immune response that will be triggered in actual human vaccine
recipients. These mAbs will also be used to create humanized animal models for more thorough vaccine
evaluation (Animal Models Core). In Aim 3, we will use our single cell immune multi-omics platform to rapidly
evaluate the immune response to sequential vaccine immunogens in near real-time, using the resulting B cell
profiles of specificity and function to inform selection of optimal candidates for subsequent boosts. Seamless
data sharing between MOVE components and sophisticated tools for visualization and analysis (Data
Management & Bioinformatics Core) will allow these analyses to occur within the normal time interval
between sequential immunizations. We can then dynamically adjust the parameters of each immunization
experiment to maximize our likelihood of success by focusing our experimental resources on the most
promising immunogen designs.

## Key facts

- **NIH application ID:** 10725054
- **Project number:** 1P01AI177683-01
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Bryan Briney
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $510,527
- **Award type:** 1
- **Project period:** 2023-06-01 → 2028-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10725054

## Citation

> US National Institutes of Health, RePORTER application 10725054, Project 2: Immune Response Analysis (1P01AI177683-01). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10725054. Licensed CC0.

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