SUMMARY/ABSTRACT: FACILITY CORE The Facility Core (FC) for the Great Lakes Center for Fresh Waters and Human Health, hosted by the University of Michigan (UM), will provide support services for four individual projects and the Community Engagement Core. The FC is divided into three subcores: nutrient analysis, led by the Stone Laboratory, Ohio State University; cyanotoxin analysis, provided by SUNY-College of Environmental Science and Forestry (ESF), University of Toledo (UT), and Wayne State University (WSU); and DNA sequencing and data synthesis at UM. Each of the participating universities has extensive experience in their respective areas of analytical expertise. All cores from the prior award period remain as members of the proposed renewal Center, which will ensure continuity in data integrity. New to this application, we have greatly expanded the mass spectrometry analysis portion of the core to include both new facilities available at SUNY-ESF and extensive new capabilities available at WSU and UT. DNA sequencing has been moved from a private company via the Administrative Core to a UM core via the FC in this renewal to better integrate data across the different projects.