# Impact of sugary drinks on gut microbiota and development of young-onset colorectal cancer

> **NIH NIH R21** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 2021 · $159,999

## Abstract

Project Summary
One of the most disturbing phenomena in colorectal cancer (CRC) in the US in the past four decades is its
increasing incidence and mortality in adults younger than 50 years old. Over the same time period, the
consumption of sugar-sweetened beverages (SSBs) has dramatically increased. Notably, more than 60% of
adolescents and young adults in the US consume at least one can (12 fl. oz.) of SSBs on a given day, suggesting
a potential link between SSBs and young-onset CRC. However, it is unclear if there are direct, causal links and
what the underlying molecular mechanisms would be. Our laboratory recently showed a direct, causal link
between SSBs and CRC using adenomatous polyposis coli (Apc)-mutant mice, which are a model for early-
stage colon adenoma (precancerous polyps). Apc-mutant mice treated with high-fructose corn syrup (HFCS, the
main sweetener of SSBs) via oral gavage for 6–8 weeks showed an increase in the total tumor number, size,
and grade, independent of obesity. Our preclinical studies suggest that consuming SSBs may shorten the time
in which benign polyps develop into CRC in humans, explaining why young-onset CRC is currently on the rise.
However, it remains unclear how HFCS does this.
Diet plays a dominant role in shaping the gut microbiome, and an unhealthy diet can lead to dysbiosis, an
imbalance in gut microbiota. A link has been shown between dysbiosis and CRC development. However, studies
on the causal and mechanistic interrelationships among diet, gut microbiota, and CRC are still lacking. We
previously observed that liquid HFCS dramatically increases the concentration of fructose (>5 mM) in the colonic
lumen in mice, which may cause dysbiosis in the colon. Furthermore, our preliminary metagenomics and
metabolomics data from wild-type mice have shown that chronic HFCS treatment increases the abundance of
specific microbes and metabolic pathways that are implicated in human CRC development. Based on these data,
we hypothesize that HFCS may facilitate CRC tumorigenesis and show the characteristics of young-onset CRC
such as mucinous and signet ring features in tumors by altering the composition and function of gut microbiota.
To test our hypothesis, we will use humanized Apc-mutant mice—in which a human microbiome has been
established through fecal microbiota transplantation—and ex vivo 3D intestinal and tumor organoids.
By completing the proposed project, we will be able to (1) determine the mechanistic and causal relationships
between sugar-induced microbiota and young-onset CRC tumorigenesis; and (2) identify sugar-specific
microbes or metabolites that can potentially be used as biomarkers and/or therapeutic targets in young-onset
CRC patients. In sum, the proposed study will significantly impact our understanding of the etiology of young-
onset CRC development and progression, potentially improving the prevention and clinical management of the
disease.

## Key facts

- **NIH application ID:** 10734338
- **Project number:** 7R21CA258134-02
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **Principal Investigator:** Jihye Yun
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $159,999
- **Award type:** 7
- **Project period:** 2021-06-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10734338

## Citation

> US National Institutes of Health, RePORTER application 10734338, Impact of sugary drinks on gut microbiota and development of young-onset colorectal cancer (7R21CA258134-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10734338. Licensed CC0.

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