PROJECT SUMMARY/ABSTRACT Cisgender Black women are frequently overlooked in HIV prevention efforts even though they have one of the highest incidences of HIV among all subpopulations, second only to men who have sex with men. Pre-exposure prophylaxis (PrEP) is a safe and effective daily medication for those at risk for HIV infection. However, data indicates a substantial inequity in PrEP use among women compared to their need. This proposed study will evaluate implementation strategies to address multi-level barriers and increase uptake of PrEP among Black cisgender women in Planned Parenthood clinics in Illinois. Our team’s preliminary work has identified successful strategies for PrEP Optimization among Women to Enhance Retention and Uptake (POWER Up). POWER Up implementation strategies address key barriers to PrEP uptake and include: 1) routine PrEP education 2) standardized provider training, 3) electronic health record (EHR) tools; and 4) PrEP navigation. These strategies have not been studied in family planning clinics with a large population of Black cisgender women. Therefore, using implementation science research methods, we propose to implement and evaluate the impact of the POWER Up strategies within the Planned Parenthood of Illinois (PPIL) health center setting to improve PrEP care continuum outcomes for Black cisgender women. By implementing POWER Up strategies in PPIL health centers we seek to address the gap in knowledge in identifying effective strategies to increase uptake of PrEP among Black cisgender women. This proposed study aims to: 1. Adapt POWER Up implementation strategies for engaging Black women in the PrEP care continuum in eight Planned Parenthood clinics in Chicago 2. Determine the effectiveness of POWER Up across eight PPIL sites within Chicago’s high prevalence HIV areas; and, 3. Assess and document implementation outcomes and report on key barriers and facilitators related to successful implementation of the intervention Findings from this study will provide insight into implementation strategies that can support effective replication across the 800 Planned Parenthood health centers and other health centers providing family planning services nationwide to reach a greater number of women at risk.