# Tissue Culture & Antibody Production Core

> **NIH NIH P01** · UT SOUTHWESTERN MEDICAL CENTER · 2024 · $324,720

## Abstract

The Tissue Culture Core (Core 1) will be responsible for providing investigators with cultured cells,
monoclonal antibodies, recombinant proteins, and recombinant adeno-associated viruses. Core 1 will be
directed by Dr. Russell DeBose-Boyd, with the assistance of Dr. Joseph Goldstein (who has directed the
Department of Molecular Genetics Tissue Culture Facility for the past 40 years) and Dr. Guosheng Liang (who
has worked in the Department for 20 years). The technical work in the Core is carried out by five experienced
technicians, one of whom (Lisa Beatty) has been in charge of this facility for more than 30 years. The physical
facilities of the Core consist of four suites of rooms that are used solely for tissue culture. The facility is equipped
with 46 incubators, 17 inverted microscopes, 1 stereo microscope, 16 sterile work areas (hoods), 2 roller bottle
apparatuses, 7 refrigerated incubator shakers, 3 table-top refrigerated centrifuges, 11 refrigerators, and 7 liquid
nitrogen freezers for storage of cell lines.
 The successful completion of this entire Program Project Grant (PPG) depends on the smooth operation
of Core 1. The Core developed considerable experience in maintaining quality control and in growing multiple
cell lines, including over 1100 different primary human fibroblast cell strains, derived from skin biopsies from
normal subjects as well as from patients with metabolic disorders supported by previous PPGs. Tens of
thousands of transfection experiments have been carried out in the Core in which various cell lines (e.g., HEK-
293, CHO, and SV589 cells) have been transfected with multiple plasmid constructs containing either the
protein-coding region or the promoter/enhancer region of multiple genes. From these transfections, more than
3000 stable and permanent cell lines have been clonally established and frozen away in multiple aliquots.
 In addition to maintenance of stock cell lines and preparation of cultured cells for experiments, the Core
is involved in the following activities: 1) Generation and maintenance of mouse and rabbit hybridoma cell lines
and production of monoclonal antibodies from culture medium; 2) Purification by Protein G- and Protein A-
Sepharose chromatography of mouse/rabbit monoclonal and rabbit polyclonal antibodies directed against
multiple proteins; 3) Growth of large volumes of suspension-culture cells that allow efficient transfection of
cDNAs and production of their encoded proteins; 4) Isolating, maintaining, and freezing away cloned cell lines
that have been transfected with mutated versions of various cDNA and promoter/enhancer constructs; 5)
Maintenance of mammalian and insect cells in suspension culture for production of recombinant proteins by
infecting these cells with recombinant baculoviruses encoding cloned cDNAs; 6) Maintenance and production
of cells for generation of recombinant adeno-associated viruses, which are used for evaluating the function of
genes in cultured cells and in the l...

## Key facts

- **NIH application ID:** 10755251
- **Project number:** 5P01HL160487-03
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** Russell Alfred DeBose-Boyd
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $324,720
- **Award type:** 5
- **Project period:** 2022-01-01 → 2026-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10755251

## Citation

> US National Institutes of Health, RePORTER application 10755251, Tissue Culture & Antibody Production Core (5P01HL160487-03). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/10755251. Licensed CC0.

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