# Embryonic stem cell/induced Pluripotent stem cell growth and gene editing core

> **NIH NIH P01** · INDIANA UNIVERSITY INDIANAPOLIS · 2024 · $467,452

## Abstract

ABSTRACT
The Embryonic Stem Cell Growth and Genetically Modified Models Core (Core B) will provide service to all four
Projects with the goal of efficiently generating and providing cellular and animal experimental models of
congenital heart disease (CHD) to Program participants. Aim 1 will provide human and mouse embryonic stem
cell (hESC and mESC)-derived cellular models of CHD to the projects. This will include (1a) providing support
for genome editing of hESC and mESCs, (1b) maintaining high quality stocks of existing and newly generated
hESC and mESC lines used by each project, and (1c) providing assistance with hESC and mESC differentiation
towards cardiac lineages. Aim 2 will provide mouse genetic models of CHD to the projects. This will include (2a)
providing support for the generation of new genetically modified mouse models, (2b) establishing and
maintaining breeding stocks for new and existing mouse models to be used by the Projects, and (2c) providing
experimental animals for each Project. Core B personnel are well-trained in all aspects of the cellular and animal
services to be provided. The goal of the Core is to provide centralized, quality-controlled reagents to the Projects,
thereby maximizing efficiency and minimizing the costs associated with the usage of cellular and animal models.
Given the heavy reliance on genetically modified models (both cellular and animal) and the proven performance
of the Core personnel, the importance of Core B to the success of this Program Project Grant application cannot
be overstated.

## Key facts

- **NIH application ID:** 10755276
- **Project number:** 5P01HL134599-07
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** Lei Yang
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $467,452
- **Award type:** 5
- **Project period:** 2017-02-15 → 2028-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10755276

## Citation

> US National Institutes of Health, RePORTER application 10755276, Embryonic stem cell/induced Pluripotent stem cell growth and gene editing core (5P01HL134599-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10755276. Licensed CC0.

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