# Cardiac Physiology and Imaging Core

> **NIH NIH P01** · INDIANA UNIVERSITY INDIANAPOLIS · 2024 · $194,708

## Abstract

Abstract
The Cardiac Physiology and Imaging Core (Core C) will provide service to all 4 projects with the goals of
generating functional and structural analyses of human Embryonic Stem Cell-derived cardiomyocytes (hESC-
CMs; Aim 1) and the adult mouse heart (Aim 2). Specifically, assays utilized in Aim 1 will provide high quality
unbiased assessment of hESC-CM structure and function. Core C’s services enhance the efficiency of the
Projects by providing consistent expertise in the functional and structural evaluation of hESC-CMs using a
variety of complementary techniques. These include confocal fluorescence microscopy for structural evaluation
as well as cytosolic [Ca2+] and membrane voltage imaging, an electrode array system for multicellular
electrophysiological and contractile assessment, Transmission Electron Microscopy (TEM) for ultrastructural
analyses, the patch-clamp technique for single hESC-CM electrophysiological measurements, and in situ
hybridization (RNAscope) for evaluation of spatial gene expression patterns. Project 1 benefits from Core C in
the analyses of gene expression at the single cell level. Projects 2 and 3 benefit from Core C in the quantitative
assessment of electrical and contractile properties of hESC-CMs. All Projects benefit from the quantitative
analysis of sarcomeric organization, polarity and shape of hESC-CMs. Techniques to be used in Aim 2 will
provide high quality unbiased functional and structural assessment of the adult mouse heart. Core C’s services
include ultra-high frequency ultrasound of mice, surface ECG in anesthetized mice, telemetric ECG monitoring
of conscious mice, optical voltage / calcium mapping of isolated mouse hearts, and quantitation of
cardiomyocyte S-phase activity using a custom-built imaging system. The Core will generate high-resolution
ultrasound images in Projects 3 in order to provide quantitative data of structure, function, and flow relative to
genetic alterations in cardiac development. ECG and voltage / calcium mapping will provide quantitation of
cardiac electrical function in Projects 2 and 3. Quantitation of cardiomyocyte S-phase activity will aid in
assessing effects of proposed genetic alterations on cardiomyocyte proliferation in all Projects.

## Key facts

- **NIH application ID:** 10755277
- **Project number:** 5P01HL134599-07
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** MICHAEL RUBART-VAN DER LOHE
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $194,708
- **Award type:** 5
- **Project period:** 2017-02-15 → 2028-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10755277

## Citation

> US National Institutes of Health, RePORTER application 10755277, Cardiac Physiology and Imaging Core (5P01HL134599-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10755277. Licensed CC0.

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